Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cho, H. T.
Right arrow Articles by Kende, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, H. T.
Right arrow Articles by Kende, H.
Agricola
Right arrow Articles by Cho, H. T.
Right arrow Articles by Kende, H.

PLANT PHYSIOLOGY , Vol 113, Issue 4 1137-1143, Copyright © 1997 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Expansins in Deepwater Rice Internodes

H. T. Cho and H. Kende
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312

Cell walls of deepwater rice (Oryza sativa L.) internodes undergo long-term extension (creep) when placed under tension in acidic buffers. This is indicative of the action of the cell wall-loosening protein expansin. Wall extension had a pH optimum of around 4.0 and was abolished by boiling. Acid-induced extension of boiled cell walls could be reconstituted by addition of salt-extracted rice or cucumber cell wall proteins. Cucumber expansin antibody recognized a single protein band of 24.5-kD apparent molecular mass on immunoblots of rice cell wall proteins. Expansins were partially purified by concanavalin A affinity chromatography and sulfopropyl (SP) cation-exchange chromatography. The latter yielded two peaks with extension activity (SP20 and SP29), and immunoblot analysis showed that both of these active fractions contained expansin of 24.5-kD molecular mass. The N-terminal amino acid sequence of SP20 expansin is identical to that deduced from the rice expansin cDNA Os-EXP1. The N-terminal amino acid sequence of SP29 expansin matches that deduced from the rice expansin cDNA Os-EXP2 in six of eight amino acids. Our results show that two expansins occur in the cell walls of rice internodes and that they may mediate acid-induced wall extension.


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Xu, J. Tian, F. C. Belanger, and B. Huang
Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species
J. Exp. Bot., October 10, 2007; (2007) erm229v1.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. OOKAWARA, S. SATOH, T. YOSHIOKA, and K. ISHIZAWA
Expression of {alpha}-Expansin and Xyloglucan Endotransglucosylase/Hydrolase Genes Associated with Shoot Elongation Enhanced by Anoxia, Ethylene and Carbon Dioxide in Arrowhead (Sagittaria pygmaea Miq.) Tubers
Ann. Bot., September 1, 2005; 96(4): 693 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Choi, Y. Lee, H.-T. Cho, and H. Kende
Regulation of Expansin Gene Expression Affects Growth and Development in Transgenic Rice Plants
PLANT CELL, June 1, 2003; 15(6): 1386 - 1398.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D.-K. Lee, J. H. Ahn, S.-K. Song, Y. D. Choi, and J. S. Lee
Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean
Plant Physiology, March 1, 2003; 131(3): 985 - 997.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
W. H. VRIEZEN, Z. ZHOU, and D. VAN DER STRAETEN
Regulation of Submergence-induced Enhanced Shoot Elongation in Oryza sativa L.
Ann. Bot., January 2, 2003; 91(2): 263 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. J. Cosgrove, L. C. Li, H.-T. Cho, S. Hoffmann-Benning, R. C. Moore, and D. Blecker
The Growing World of Expansins
Plant Cell Physiol., December 15, 2002; 43(12): 1436 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Yuan, Y. Wu, and D. J. Cosgrove
A Fungal Endoglucanase with Plant Cell Wall Extension Activity
Plant Physiology, September 1, 2001; 127(1): 324 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Wu, R. B. Meeley, and D. J. Cosgrove
Analysis and Expression of the {alpha}-Expansin and {beta}-Expansin Gene Families in Maize
Plant Physiology, May 1, 2001; 126(1): 222 - 232.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. K.C. Rose, D. J. Cosgrove, P. Albersheim, A. G. Darvill, and A. B. Bennett
Detection of Expansin Proteins and Activity during Tomato Fruit Ontogeny
Plant Physiology, August 1, 2000; 123(4): 1583 - 1592.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
H. Kende, E. van der Knaap, and H.-T. Cho
Deepwater Rice: A Model Plant to Study Stem Elongation
Plant Physiology, December 1, 1998; 118(4): 1105 - 1110.
[Full Text]

Home page
 Plant Physiol.Home page
D. J. Cosgrove
Cell Wall Loosening by Expansins
Plant Physiology, October 1, 1998; 118(2): 333 - 339.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. J. Cosgrove, P. Bedinger, and D. M. Durachko
Group I allergens of grass pollen as cell wall-loosening agents
PNAS, June 10, 1997; 94(12): 6559 - 6564.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists