Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Makino, A.
Right arrow Articles by Yamamoto, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Makino, A.
Right arrow Articles by Yamamoto, N.
Agricola
Right arrow Articles by Makino, A.
Right arrow Articles by Yamamoto, N.

PLANT PHYSIOLOGY , Vol 114, Issue 2 483-491, Copyright © 1997 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Does Decrease in Ribulose-1,5-Bisphosphate Carboxylase by Antisense RbcS Lead to a Higher N-Use Efficiency of Photosynthesis under Conditions of Saturating CO2 and Light in Rice Plants?

A. Makino, T. Shimada, S. Takumi, K. Kaneko, M. Matsuoka, K. Shimamoto, H. Nakano, M. Miyao-Tokutomi, T. Mae and N. Yamamoto
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Tsutsumidori-Amamiyamachi, Sendai 981, Japan (A.M., K.K., H.N., T.M.)

Rice (Oryza sativa L.) plants with decreased ribulose-1,5-bisphosphate carboxylase (Rubisco) were obtained by transformation with the rice rbcS antisense gene under the control of the rice rbcS promoter. The primary transformants were screened for the Rubisco to leaf N ratio, and the transformant with 65% wild-type Rubisco was selected as a plant set with optimal Rubisco content at saturating CO2 partial pressures for photosynthesis under conditions of high irradiance and 25[deg]C. This optimal Rubisco content was estimated from the amounts and kinetic constants of Rubisco and the gas-exchange data. The R1 selfed progeny of the selected transformant were grown hydroponically with different N concentrations. Rubisco content in the R1 population was distributed into two groups: 56 plants had about 65% wild-type Rubisco, whereas 23 plants were very similar to the wild type. Although the plants with decreased Rubisco showed 20% lower rates of light-saturated photosynthesis in normal air (36 Pa CO2), they had 5 to 15% higher rates of photosynthesis in elevated partial pressures of CO2, (100-115 Pa CO2) than the wild-type plants for a given leaf N content. We conclude that the rice plants with 65% wild-type Rubisco show a higher N-use efficiency of photosynthesis under conditions of saturating CO2 and high irradiance.


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
K. Imai, Y. Suzuki, T. Mae, and A. Makino
Changes in the Synthesis of Rubisco in Rice Leaves in Relation to Senescence and N Influx
Ann. Bot., January 1, 2008; 101(1): 135 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Makino and R. F. Sage
Temperature Response of Photosynthesis in Transgenic Rice Transformed with 'Sense' or 'Antisense' rbcS
Plant Cell Physiol., October 1, 2007; 48(10): 1472 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Suzuki, M. Ohkubo, H. Hatakeyama, K. Ohashi, R. Yoshizawa, S. Kojima, T. Hayakawa, T. Yamaya, T. Mae, and A. Makino
Increased Rubisco Content in Transgenic Rice Transformed with the 'Sense' rbcS Gene
Plant Cell Physiol., April 1, 2007; 48(4): 626 - 637.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
I. TERASHIMA, T. ARAYA, S.-I. MIYAZAWA, K. SONE, and S. YANO
Construction and Maintenance of the Optimal Photosynthetic Systems of the Leaf, Herbaceous Plant and Tree: an Eco-developmental Treatise
Ann. Bot., February 1, 2005; 95(3): 507 - 519.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Matsuda, K. Ohashi-Kaneko, K. Fujiwara, E. Goto, and K. Kurata
Photosynthetic Characteristics of Rice Leaves Grown under Red Light with or without Supplemental Blue Light
Plant Cell Physiol., December 15, 2004; 45(12): 1870 - 1874.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Hirotsu, A. Makino, A. Ushio, and T. Mae
Changes in the Thermal Dissipation and the Electron Flow in the Water-Water Cycle in Rice Grown Under Conditions of Physiologically Low Temperature
Plant Cell Physiol., May 15, 2004; 45(5): 635 - 644.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Makino, H. Sakuma, E. Sudo, and T. Mae
Differences between Maize and Rice in N-use Efficiency for Photosynthesis and Protein Allocation
Plant Cell Physiol., September 15, 2003; 44(9): 952 - 956.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. M. Whitney and T. J. Andrews
Photosynthesis and Growth of Tobacco with a Substituted Bacterial Rubisco Mirror the Properties of the Introduced Enzyme
Plant Physiology, September 1, 2003; 133(1): 287 - 294.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. J. Parry, P. J. Andralojc, R. A. C. Mitchell, P. J. Madgwick, and A. J. Keys
Manipulation of Rubisco: the amount, activity, function and regulation
J. Exp. Bot., May 1, 2003; 54(386): 1321 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. H. Murchie, S. Hubbart, Y. Chen, S. Peng, and P. Horton
Acclimation of Rice Photosynthesis to Irradiance under Field Conditions
Plant Physiology, December 1, 2002; 130(4): 1999 - 2010.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. H. Murchie, J. Yang, S. Hubbart, P. Horton, and S. Peng
Are there associations between grain-filling rate and photosynthesis in the flag leaves of field-grown rice?
J. Exp. Bot., November 1, 2002; 53(378): 2217 - 2224.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Makino, C. Miyake, and A. Yokota
Physiological Functions of the Water-Water Cycle (Mehler Reaction) and the Cyclic Electron Flow around PSI in Rice Leaves
Plant Cell Physiol., September 15, 2002; 43(9): 1017 - 1026.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Ishimaru, N. Kobayashi, K. Ono, M. Yano, and R. Ohsugi
Are contents of Rubisco, soluble protein and nitrogen in flag leaves of rice controlled by the same genetics?
J. Exp. Bot., September 1, 2001; 52(362): 1827 - 1833.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Makino, H. Nakano, T. Mae, T. Shimada, and N. Yamamoto
Photosynthesis, plant growth and N allocation in transgenic rice plants with decreased Rubisco under CO2 enrichment
J. Exp. Bot., February 1, 2000; 51(90001): 383 - 389.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
R.A.C. Mitchell, J.C. Theobald, M.A.J. Parry, and D.W. Lawlor
Is there scope for improving balance between RuBP-regeneration and carboxylation capacities in wheat at elevated CO2?
J. Exp. Bot., February 1, 2000; 51(90001): 391 - 397.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
H. Eichelmann and A. Laisk
Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Content, Assimilatory Charge, and Mesophyll Conductance in Leaves
Plant Physiology, January 1, 1999; 119(1): 179 - 190.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. C. Theobald, R. A.C. Mitchell, M. A.J. Parry, and D. W. Lawlor
Estimating the Excess Investment in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Leaves of Spring Wheat Grown under Elevated CO2
Plant Physiology, November 1, 1998; 118(3): 945 - 955.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists