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PLANT PHYSIOLOGY , Vol 114, Issue 3 1071-1076, Copyright © 1997 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
A cDNA Clone Highly Expressed in Ripe Banana Fruit Shows Homology to Pectate Lyases
E. Dominguez-Puigjaner, I. Llop, M. Vendrell and S. Prat
Departmento de Agrobiologia (E.D.-P., I.L., M.V.), and Departmento de Genetica Molecular (S.P.), Centro de Investigacion y Desarrollo-Consejo Superior de Investigaciones Cientificas, Jorge Girona, 18-26 08034 Barcelona, Spain
A cDNA clone (Banl7), encoding a protein homologous to pectate lyase, has
been isolated from a cDNA library from climacteric banana fruit by means of
differential screening. Northern analysis showed that Banl7 mRNA is first
detected in early climacteric fruit, reaches a steady-state maximum at the
climacteric peak, and declines thereafter in overripe fruit. Accumulation
of the Banl7 transcript can be induced in green banana fruit by exogenous
application of ethylene. This demonstrates that expression of this gene is
under hormonal control, its induction being regulated by the rapid increase
in ethylene production at the onset of ripening. The deduced amino acid
sequence derived from the Banl7 cDNA shares significant identity with
pectate lyases from pollen and plant pathogenic bacteria of the genus
Erwinia. Similarity to bacterial pectate lyases that were proven to break
down the pectic substances of the plant cell wall suggest that Banl7 might
play a role in the loss of mesocarp firmness during fruit ripening.
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