Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, W.
Right arrow Articles by Stacey, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, W.
Right arrow Articles by Stacey, G.
Agricola
Right arrow Articles by Song, W.
Right arrow Articles by Stacey, G.

PLANT PHYSIOLOGY , Vol 114, Issue 3 927-935, Copyright © 1997 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

W. Song, S. Koh, M. Czako, L. Marton, E. Drenkard, J. M. Becker and G. Stacey
Center for Legume Research (W.S., S.K., G.S.), Department of Microbiology (W.S., S.K., J.M.B., G.S.), and Ecology and Evolutionary Biology (G.S.), The University of Tennessee, Knoxville, Tennessee 37996-0845

Previously, we identified a peptide transport gene, AtPTR2-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AtPTR2-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTR2-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AtPTR2-B mRNA levels were significantly reduced in transgenic leaves and flowers, but not in transgenic roots. Consistent with this reduction in endogenous AtPTR2-B mRNA levels, all four antisense lines and one sense line exhibited significant phenotypic changes, including late flowering and arrested seed development. These phenotypic changes could be explained by a defect in nitrogen nutrition due to the reduced peptide transport activity conferred by AtPTR2-B. These results suggest that AtPTR2-B may play a general role in plant nutrition. The AtPTR2-B gene was mapped to chromosome 2, which is closely linked to the restriction fragment length polymorphism marker m246.


This article has been cited by other articles:


Home page
 Plant CellHome page
A. Almagro, S. H. Lin, and Y. F. Tsay
Characterization of the Arabidopsis Nitrate Transporter NRT1.6 Reveals a Role of Nitrate in Early Embryo Development
PLANT CELL, December 1, 2008; 20(12): 3289 - 3299.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Y. Komarova, K. Thor, A. Gubler, S. Meier, D. Dietrich, A. Weichert, M. Suter Grotemeyer, M. Tegeder, and D. Rentsch
AtPTR1 and AtPTR5 Transport Dipeptides in Planta
Plant Physiology, October 1, 2008; 148(2): 856 - 869.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
W. M. WATERWORTH and C. M. BRAY
Enigma Variations for Peptides and Their Transporters in Higher Plants
Ann. Bot., July 1, 2006; 98(1): 1 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C.-C. Chiu, C.-S. Lin, A.-P. Hsia, R.-C. Su, H.-L. Lin, and Y.-F. Tsay
Mutation of a Nitrate Transporter, AtNRT1:4, Results in a Reduced Petiole Nitrate Content and Altered Leaf Development
Plant Cell Physiol., September 15, 2004; 45(9): 1139 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Cagnac, A. Bourbouloux, D. Chakrabarty, M.-Y. Zhang, and S. Delrot
AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron
Plant Physiology, July 1, 2004; 135(3): 1378 - 1387.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W.-R. Scheible, B. Fry, A. Kochevenko, D. Schindelasch, L. Zimmerli, S. Somerville, R. Loria, and C. R. Somerville
An Arabidopsis Mutant Resistant to Thaxtomin A, a Cellulose Synthesis Inhibitor from Streptomyces Species
PLANT CELL, August 1, 2003; 15(8): 1781 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Miranda, L. Borisjuk, A. Tewes, D. Dietrich, D. Rentsch, H. Weber, and U. Wobus
Peptide and Amino Acid Transporters Are Differentially Regulated during Seed Development and Germination in Faba Bean
Plant Physiology, August 1, 2003; 132(4): 1950 - 1960.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. G. Stacey, S. Koh, J. Becker, and G. Stacey
AtOPT3, a Member of the Oligopeptide Transporter Family, Is Essential for Embryo Development in Arabidopsis
PLANT CELL, November 1, 2002; 14(11): 2799 - 2811.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Koh, A. M. Wiles, J. S. Sharp, F. R. Naider, J. M. Becker, and G. Stacey
An Oligopeptide Transporter Gene Family in Arabidopsis
Plant Physiology, January 1, 2002; 128(1): 21 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. M. Waterworth, C. E. West, and C. M. Bray
The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane
J. Exp. Bot., July 1, 2000; 51(348): 1201 - 1209.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists