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PLANT PHYSIOLOGY , Vol 115, Issue 1 213-222, Copyright © 1997 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Structure and Blue-Light-Responsive Transcription of a Chloroplast psbD Promoter from Arabidopsis thaliana
P. H. Hoffer and D. A. Christopher
Department of Plant Molecular Physiology, University of Hawaii at Manoa, St. John 503, 3190 Maile Way, Honolulu, Hawaii 96822
We characterized the effects of light on psbD transcription and mRNA levels
during chloroplast development in Arabidopsis thaliana. After 6 to 12 hours
of illumination of dark-grown seedlings, two psbD mRNAs were detected and
their 5[prime] ends were mapped to positions -550 and -190 bp upstream from
the psbD translational start codon. Their kinetics of accumulation
resembled the accumulation of chloroplast psbA and rbcL mRNAs but differed
from the accumulation of the nuclear-encoded Lhcb and Chs mRNAs. A third
psbD mRNA with its 5[prime] end at position -950 accumulated after
illumination of > 180 h. The 5[prime] ends of this transcript were
mapped to a nucleotide sequence that is highly conserved with functional
sequences in the barley (Hordeum vulgare) blue-light-responsive promoter
(BLRP). Transcription from the Arabidopsis psbD promoter was 3-fold higher
in blue relative to red light, whereas red and blue light affected total
chloroplast, rbcL, and 16S rDNA transcription similarly. This study shows
that transcription of Arabidopsis psbD is mediated by a BLRP and suggests
that psbD genes in other land plants are regulated by a common
blue-light-signaling pathway. Isolating the BLRP from Arabidopsis will
allow molecular genetic studies aimed at identifying the pertinent
photoreceptor and components of this phototransduction pathway.
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