Plant Physiol. EPICENTRE Biotechnologies
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, Z. B.
Right arrow Articles by Guilfoyle, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Z. B.
Right arrow Articles by Guilfoyle, T. J.
Agricola
Right arrow Articles by Liu, Z. B.
Right arrow Articles by Guilfoyle, T. J.

PLANT PHYSIOLOGY , Vol 115, Issue 2 397-407, Copyright © 1997 by American Society of Plant Biologists

GENE REGULATION AND MOLECULAR GENETICS

A G-Box-Binding Protein from Soybean Binds to the E1 Auxin-Response Element in the Soybean GH3 Promoter and Contains a Proline-Rich Repression Domain

Z. B. Liu, G. Hagen and T. J. Guilfoyle
Department of Biochemistry, 117 Schweitzer Hall, University of Missouri, Columbia, Missouri 65211

The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plants, as well as in transfected protoplasts. The AuxRE within E1 contains a G-box, and this G-box was used to clone a G-box-binding factor (GBF) from soybean (SGBF-2). This 45-kD GBF contains an N-terminal proline-rich domain and a C-terminal basic/leucine zipper DNA-binding domain. Gel-mobility shift assays were used to characterize the binding specificity of SGBF-2. Antiserum raised against recombinant SGBF-2 was used to further characterize SGBF-2 and antigenically related GBFs in soybean nuclear extracts. Co-transfection assays with effector and reporter plasmids in carrot (Daucus carota L.) protoplasts indicated that the N-terminal proline-rich domain of SGBF-2 functioned as a repression domain in both basal and auxin-inducible transcription.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. S. Kim, B. O. Park, J. H. Yoo, M. S. Jung, S. M. Lee, H. J. Han, K. E. Kim, S. H. Kim, C. O. Lim, D.-J. Yun, et al.
Identification of a Calmodulin-binding NAC Protein as a Transcriptional Repressor in Arabidopsis
J. Biol. Chem., December 14, 2007; 282(50): 36292 - 36302.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Tamai, M. Iwabuchi, and T. Meshi
Arabidopsis GARP Transcriptional Activators Interact with the Pro-Rich Activation Domain Shared by G-Box-Binding bZIP Factors
Plant Cell Physiol., January 1, 2002; 43(1): 99 - 107.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Song, H. Solimeo, R. A. Rupert, N. S. Yadav, and Q. Zhu
Functional Dissection of a Rice Dr1/DrAp1 Transcriptional Repression Complex
PLANT CELL, January 1, 2002; 14(1): 181 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Ohta, K. Matsui, K. Hiratsu, H. Shinshi, and M. Ohme-Takagi
Repression Domains of Class II ERF Transcriptional Repressors Share an Essential Motif for Active Repression
PLANT CELL, August 1, 2001; 13(8): 1959 - 1968.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Y. Fujimoto, M. Ohta, A. Usui, H. Shinshi, and M. Ohme-Takagi
Arabidopsis Ethylene-Responsive Element Binding Factors Act as Transcriptional Activators or Repressors of GCC Box-Mediated Gene Expression
PLANT CELL, March 1, 2000; 12(3): 393 - 404.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ulmasov, G. Hagen, and T. J. Guilfoyle
Activation and repression of transcription by auxin-response factors
PNAS, May 11, 1999; 96(10): 5844 - 5849.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Guilfoyle, G. Hagen, T. Ulmasov, and J. Murfett
How Does Auxin Turn On Genes?
Plant Physiology, October 1, 1998; 118(2): 341 - 347.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists