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PLANT PHYSIOLOGY , Vol 115, Issue 3 1175-1184, Copyright © 1997 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Changes in the Composition of the Photosynthetic Apparatus in the Galactolipid-Deficient dgd1 Mutant of Arabidopsis thaliana
H. Hartel, H. Lokstein, P. Dormann, B. Grimm and C. Benning
Institut fur Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195 Berlin, Germany (H.H., P.D., C.B.)
The glycerolipid digalactosyl diacylglycerol (DGDG) is exclusively
associated with photosynthetic membranes and thus may play a role in the
proper assembly and maintenance of the photosynthetic apparatus. Here we
employ a genetic approach based on the dgd1 mutant of Arabidopsis thaliana
to investigate the function of DGDG in thylakoid membranes. The primary
defect in the genetically well-characterized dgd1 mutant resulted in a 90%
reduction of the DGDG content. The mutant showed a decreased photosystem II
(PSII) to photosystem I ratio. In vivo room- and low-temperature (77 K)
chlorophyll fluorescence measurements with thylakoid preparations are in
agreement with a drastically altered excitation energy allocation to the
reaction centers. Quantification of pigment-binding apoproteins and
pigments supports an altered stoichiometry of individual pigment-protein
complexes in the mutant. Most strikingly, an increase in the amount of
peripheral light-harvesting complexes of PSII relative to the inner antenna
complexes and the PSII reaction center/core complexes was observed.
Regardless of the severe alterations in thylakoid organization,
photosynthetic oxygen evolution was virtually not compromised in dgd1
mutant leaves.
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