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PLANT PHYSIOLOGY , Vol 115, Issue 3 925-937, Copyright © 1997 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Developmental and Hormonal Regulation of the Arabidopsis CER2 Gene That Codes for a Nuclear-Localized Protein Required for the Normal Accumulation of Cuticular Waxes
Y. Xia, B. J. Nikolau and P. S. Schnable
Departments of Zoology and Genetics (Y.X., P.S.S.), Biochemistry and Biophysics (B.J.N.), and Agronomy (P.S.S.), Iowa State University, Ames, Iowa 50011
The previously cloned CER2 gene is required for the normal accumulation of
cuticular waxes and encodes a novel protein. Earlier reports suggested that
the CER2 protein is either a membrane-bound component of the fatty acid
elongase complex or a regulatory protein. Cell fractionation and immunoblot
analyses using polyclonal antibodies raised against a chemically
synthesized peptide with a sequence based on the predicted CER2 protein
sequence have demonstrated that the 47-kD CER2 protein is soluble and
nuclear localized. These results are consistent with CER2 being a
regulatory protein. Detailed studies of plants harboring a CER2
promoter/GUS transgene (CER2-GUS), in combination with immunoblot analyses,
revealed that CER2 is expressed and the CER2 protein accumulates in a
variety of organs and cell types. Expression is highest early in the
development of these organs and is epidermis specific in most tissues. In
agreement with the activity of the CER2 promoter in hypocotyls, cuticular
wax accumulates on this organ in a CER2-dependent fashion. In leaves CER2
expression is confined to the guard cells, trichomes, and petioles.
However, application of the cytokinin 6-benzylaminopurine induces ectopic
expression of CER2-GUS in all cell types of leaves that emerge following
treatment.
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