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PLANT PHYSIOLOGY , Vol 115, Issue 4 1405-1412, Copyright © 1997 by American Society of Plant Biologists
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WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Flavonoid-Peroxidase Reaction as a Detoxification Mechanism of Plant Cells against H2O2
H. Yamasaki, Y. Sakihama and N. Ikehara
Laboratory of Cell and Functional Biology, College of Science, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
Recent studies have revealed that dietary flavonoids are potent radical
scavengers, acting in a manner similar to ascorbate and [alpha]-tocopherol.
However, it is still not clear whether flavonoids have a similar
antioxidative function in plants. We examined the possibility that
flavonoids could function as stress protectants in plant cells by
scavenging H2O2. Two major flavonoids, quercetin and kaempferol glycosides,
were isolated from leaves of the tropical tree Schefflera arboricola
Hayata. Both glycosides and aglycones of isolated flavonols were oxidized
by H2O2 in the presence of horse-radish peroxidase and/or in a soluble
fraction of S. arboricola leaf extract. The rates of oxidation were in the
order quercetin > kaempferol > quercetin glycoside >>
kaempferol glycoside. Judging from the effects of inhibitors such as KCN,
p-chloromercuribenzoate, and 3-amino-1H-1,2,4-triazole, we conclude that
guaiacol peroxidase in the soluble fraction catalyzes H2O2-dependent
oxidation of flavonols. In the flavonol-guaiacol peroxidase reaction,
ascorbate had the potential to regenerate flavonols by reducing the
oxidized product. These results provide further evidence that the
flavonoid-peroxidase reaction can function as a mechanism for H2O2
scavenging in plants.
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