Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mock, H.-P.
Right arrow Articles by Grimm, B.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Mock, H.-P.
Right arrow Articles by Grimm, B.
Agricola
Right arrow Articles by Mock, H.-P.
Right arrow Articles by Grimm, B.

Defense Responses to Tetrapyrrole-Induced Oxidative Stress in Transgenic Plants with Reduced Uroporphyrinogen Decarboxylase or Coproporphyrinogen Oxidase Activity1

Hans-Peter Mock, Ulrich Keetman, Elisabeth Kruse, Barbara Rank, and Bernhard Grimm*

Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany (H.-P.M., U.K., E.K., B.G.); and Institut für Biologie, Humboldt-Universität zu Berlin, Unter den Linden 6 (Sitz:Philippstrasse 13), D-10099 Berlin, Germany (U.K., B.R.)

We analyzed the antioxidative defense responses of transgenic tobacco (Nicotiana tabacum) plants expressing antisense RNA for uroporphyrinogen decarboxylase or coproporphyrinogen oxidase. These plants are characterized by necrotic leaf lesions resulting from the accumulation of potentially photosensitizing tetrapyrroles. Compared with control plants, the transformants had increased levels of antioxidant mRNAs, particularly those encoding superoxide dismutase (SOD), catalase, and glutathione peroxidase. These elevated transcript levels correlated with increased activities of cytosolic Cu/Zn-SOD and mitochondrial Mn-SOD. Total catalase activity decreased in the older leaves of the transformants to levels lower than in the wild-type plants, reflecting an enhanced turnover of this photosensitive enzyme. Most of the enzymes of the Halliwell-Asada pathway displayed increased activities in transgenic plants. Despite the elevated enzyme activities, the limited capacity of the antioxidative system was apparent from decreased levels of ascorbate and glutathione, as well as from necrotic leaf lesions and growth retardation. Our data demonstrate the induction of the enzymatic detoxifying defense system in several compartments, suggesting a photosensitization of the entire cell. It is proposed that the tetrapyrroles that initially accumulate in the plastids leak out into other cellular compartments, thereby necessitating the local detoxification of reactive oxygen species.

1   This work was partially supported by grants from the Deutsche Forschungsgemeinschaft (nos. 936/3-1 and 936/4-1).
*   Corresponding author; e-mail grimm{at}ipk-gatersleben.de; fax 49-39-482-5136.

Plant Physiol. (1998) 116: 107-116
Copyright Clearance Center:   0032-0889/98/116/0107/10
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Pollmann, A. Springer, F. Buhr, A. Lahroussi, I. Samol, J.-M. Bonneville, G. Tichtinsky, D. von Wettstein, C. Reinbothe, and S. Reinbothe
A plant porphyria related to defects in plastid import of protochlorophyllide oxidoreductase A
PNAS, February 6, 2007; 104(6): 2019 - 2023.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Zimmermann, H. Baumlein, H.-P. Mock, A. Himmelbach, and P. Schweizer
The Multigene Family Encoding Germin-Like Proteins of Barley. Regulation and Function in Basal Host Resistance
Plant Physiology, September 1, 2006; 142(1): 181 - 192.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Gray, D. Janick-Buckner, B. Buckner, P. S. Close, and G. S. Johal
Light-Dependent Death of Maize lls1 Cells Is Mediated by Mature Chloroplasts
Plant Physiology, December 1, 2002; 130(4): 1894 - 1907.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Meskauskiene, M. Nater, D. Goslings, F. Kessler, R. op den Camp, and K. Apel
FLU: A negative regulator of chlorophyll biosynthesis in Arabidopsisthaliana
PNAS, October 12, 2001; (2001) 221252798.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-P. Mock, W. Heller, A. Molina, B. Neubohn, H. Sandermann Jr., and B. Grimm
Expression of Uroporphyrinogen Decarboxylase or Coproporphyrinogen Oxidase Antisense RNA in Tobacco Induces Pathogen Defense Responses Conferring Increased Resistance to Tobacco Mosaic Virus
J. Biol. Chem., February 12, 1999; 274(7): 4231 - 4238.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. B. Taylor
Vampire Plants?
PLANT CELL, July 1, 1998; 10(7): 1071 - 1074.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Meskauskiene, M. Nater, D. Goslings, F. Kessler, R. op den Camp, and K. Apel
FLU: A negative regulator of chlorophyll biosynthesis in Arabidopsisthaliana
PNAS, October 23, 2001; 98(22): 12826 - 12831.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists