Co-Permeability of ³H-Labeled Water and ¹⁴C-Labeled Organic Acids across Isolated Plant Cuticles¹
Investigating Cuticular Paths of Diffusion and Predicting Cuticular Transpiration

Sabine Niederl, Thomas Kirsch, Markus Riederer, and Lukas Schreiber^*

Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany

Penetration of ³H-labeled water (³H₂O) and the ¹⁴C-labeled organic acids benzoic acid ([¹⁴C]BA), salicylic acid ([¹⁴C]SA), and 2,4-dichlorophenoxyacetic acid ([¹⁴C]2,4-D) were measured simultaneously in isolated cuticular membranes of Prunus laurocerasus L., Ginkgo biloba L., and Juglans regia L. For each of the three pairs of compounds (³H₂O/[¹⁴C]BA, ³H₂O/[¹⁴C]SA, and ³H₂O/[¹⁴C]2,4-D) rates of cuticular water penetration were highly correlated with the rates of penetration of the organic acids. Therefore, water and organic acids penetrated the cuticles by the same routes. With the combination ³H₂O/[¹⁴C]BA, co-permeability was measured with isolated cuticles of nine other plant species. Permeances of ³H₂O of all 12 investigated species were highly correlated with the permeances of [¹⁴C]BA (r² = 0.95). Thus, cuticular transpiration can be predicted from BA permeance. The application of this experimental method, together with the established prediction equation, offers the opportunity to answer several important questions about cuticular transport physiology in future investigations.

Plant Physiol. (1998) 116: 117-123
Copyright Clearance Center:   0032-0889/98/116/0117/07
© 1998 American Society of Plant Physiologists

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