Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hyun Lee, S.
Right arrow Articles by Sung Lee, W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hyun Lee, S.
Right arrow Articles by Sung Lee, W.
Agricola
Right arrow Articles by Hyun Lee, S.
Right arrow Articles by Sung Lee, W.

Ethylene-Mediated Phospholipid Catabolic Pathway in Glucose-Starved Carrot Suspension Cells1

Soo Hyun Lee, Hyun Sook Chae, Taek Kyun Lee, Se Hee Kim, Sung Ho Shin, Bong Huey Cho, Sung Ho Cho, Bin G. Kang, and Woo Sung Lee*

Department of Biology, Sung Kyun Kwan University, Suwon 440-746, Korea (S.H.L., T.K.L., S.H.K., W.S.L.); Department of Biology, Yonsei University, Seoul 120-749, Korea (H.S.C., B.G.K.); Agency for Defense Development, Yuseong 305-600, Korea (S.H.S.); Department of Biology, Suwon University, Suwon 445-743, Korea (B.H.C.); and Department of Biology, Inha University, Inchon 402-751, Korea (S.H.C.)

Glucose (Glc) starvation of suspension-cultured carrot (Daucus carota L.) cells resulted in sequential activation of phospholipid catabolic enzymes. Among the assayed enzymes involved in the degradation, phospholipase D (PLD) and lipolytic acyl hydrolase were activated at the early part of starvation, and these activities were followed by beta -oxidation and the glyoxylate cycle enzymes in order. The activity of PLD and lipolytic acyl hydrolase was further confirmed by in vivo-labeling experiments. It was demonstrated that Glc added to a medium containing starving cells inhibited the phospholipid catabolic activities, indicating that phospholipid catabolism is negatively regulated by Glc. There was a burst of ethylene production 6 h after starvation. Ethylene added exogeneously to a Glc-sufficient medium activated PLD, indicating that ethylene acts as an element in the signal transduction pathway leading from Glc depletion to PLD activation. Activation of lipid peroxidation, suggestive of cell death, occurred immediately after the decrease of the phospholipid degradation, suggesting that the observed phospholipid catabolic pathway is part of the metabolic strategies by which cells effectively survive under Glc starvation.

1   This work was supported by the Academic Research Fund (GE 96-212) of the Ministry of Education, Republic of Korea, awarded to W.S.L. This work was also partly supported by a grant from Korea Science and Engineering Foundation-Hormone Research Center (97-K-3-0401-03) awarded to B.G.K.
*   Corresponding author; e-mail wslee{at}yurim.skku.ac.kr; fax 82-331-290-7015.

Plant Physiol. (1998) 116: 223-229
Copyright Clearance Center:   0032-0889/98/116/0223/07
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Malladi and J. K. Burns
CsPLD{alpha}1 and CsPLD{gamma}1 are differentially induced during leaf and fruit abscission and diurnally regulated in Citrus sinensis
J. Exp. Bot., October 1, 2008; 59(13): 3729 - 3739.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. E. McLAUGHLIN and J. S. BOYER
Sugar-responsive Gene Expression, Invertase Activity, and Senescence in Aborting Maize Ovaries at Low Water Potentials
Ann. Bot., November 1, 2004; 94(5): 675 - 689.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Munne-Bosch, J. Penuelas, D. Asensio, and J. Llusia
Airborne Ethylene May Alter Antioxidant Protection and Reduce Tolerance of Holm Oak to Heat and Drought Stress
Plant Physiology, October 1, 2004; 136(2): 2937 - 2947.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Sang, D. Cui, and X. Wang
Phospholipase D and Phosphatidic Acid-Mediated Generation of Superoxide in Arabidopsis
Plant Physiology, August 1, 2001; 126(4): 1449 - 1458.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Manoharan, H. S. Chae, J. Myoung, S. H. Cho, S. H. Shin, B. H. Cho, and W. S. Lee
Synthesis of Phosphatidylserine in Carrot Cells Cultured under Carbon-Source Starvation
Plant Cell Physiol., October 1, 2000; 41(10): 1143 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Poirier, G. Ventre, and D. Caldelari
Increased Flow of Fatty Acids toward beta -Oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-Chain-Length Fatty Acids
Plant Physiology, December 1, 1999; 121(4): 1359 - 1366.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
X. Wang
The Role of Phospholipase D in Signaling Cascades
Plant Physiology, July 1, 1999; 120(3): 645 - 652.
[Full Text]

Home page
 Plant Physiol.Home page
L. Fan, S. Zheng, D. Cui, and X. Wang
Subcellular Distribution and Tissue Expression of Phospholipase Dalpha , Dbeta , and Dgamma in Arabidopsis
Plant Physiology, April 1, 1999; 119(4): 1371 - 1378.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists