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Metabolism of Polyamines in Transgenic Cells of Carrot Expressing a Mouse Ornithine Decarboxylase cDNA1

Scott E. Andersen2, Dhundy R. Bastola3, and Subhash C. Minocha*

Department of Plant Biology, University of New Hampshire, Durham, New Hampshire 03824

The metabolisms of arginine (Arg), ornithine (Orn), and putrescine were compared in a nontransgenic and a transgenic cell line of carrot (Daucus carota L.) expressing a mouse Orn decarboxylase cDNA. [14C]Arg, [14C]Orn, and [14C]putrescine were fed to cells and their rates of decarboxylation, uptake, metabolism into polyamines, and incorporation into acid-insoluble material were determined. Transgenic cells showed higher decarboxylation rates for labeled Orn than the nontransgenic cells. This was correlated positively with higher amounts of labeled putrescine production from labeled Orn. With labeled Arg, both the transgenic and the nontransgenic cells exhibited similar rates of decarboxylation and conversion into labeled putrescine. When [14C]putrescine was fed, higher rates of degradation were observed in transgenic cells as compared with the nontransgenic cells. It is concluded that (a) increased production of putrescine via the Orn decarboxylase pathway has no compensatory effects on the Arg decarboxylase pathway, and (b) higher rates of putrescine production in the transgenic cells are accompanied by higher rates of putrescine conversion into spermidine and spermine as well as the catabolism of putrescine.

1   This is scientific contribution no. 1959 from the New Hampshire Agricultural Experiment Station.
2   Present address: Gene Discovery and Expression, Monsanto, St. Louis, MO 63042.
3   Present address: Department of Biochemistry, School of Medicine, University of Nevada, Reno, NV 89577.
*   Corresponding author; e-mail sminocha{at}christa.unh.edu; fax 1-603-862-3784.

Plant Physiol. (1998) 116: 299-307
Copyright Clearance Center:   0032-0889/98/116/0299/09
© 1998 American Society of Plant Physiologists




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