Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Characterization of DNA-Binding Proteins from Pea Mitochondria1

Frank Hatzack2, Saskia Dombrowski, Axel Brennicke, and Stefan Binder*

Allgemeine Botanik, Universität Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany

We studied transcription initiation in the mitochondria of higher plants, with particular respect to promoter structures. Conserved elements of these promoters have been successfully identified by in vitro transcription systems in different species, whereas the involved protein components are still unknown. Proteins binding to double-stranded oligonucleotides representing different parts of the pea (Pisum sativum) mitochondrial atp9 were analyzed by denaturation-renaturation chromatography and mobility-shift experiments. Two DNA-protein complexes were detected, which appeared to be sequence specific in competition experiments. Purification by hydroxyapatite, phosphocellulose, and reversed-phase high-pressure liquid chromatography separated two polypeptides with apparent molecular masses of 32 and 44 kD. Both proteins bound to conserved structures of the pea atp9 and the heterologous Oenothera berteriana atp1 promoters and to sequences just upstream. Possible functions of these proteins in mitochondrial promoter recognition are discussed.

1   This work was supported by the Deutsche Forschungsgemeinschaft, a Landesforschungsschwerpunkt Baden-Württemberg, the Human Frontiers Science Program, and the Fonds der Chemischen Industrie.
2   Present address: Plant Genetics, Environmental Sciences and Technology Department, Risø National Laboratory, DK-4000 Roskilde, Denmark.
*   Corresponding author; e-mail stefan.binder{at}biologie.uni-ulm.de; fax 49-731-502-2626.

Plant Physiol. (1998) 116: 519-527
Copyright Clearance Center:   0032-0889/98/116/0519/09
© 1998 American Society of Plant Physiologists




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