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Stress Induction of Mitochondrial Formate Dehydrogenase in Potato
Leaves1
Cécile Hourton-Cabassa,
Françoise Ambard-Bretteville,
François Moreau,
Jacques Davy de Virville,
René Rémy, and
Catherine Colas des Francs-Small*
Institut de Biotechnologie des Plantes, Centre National de la
Recherche Scientifique-Equipe en Restructuration 569 Université
Paris-Sud, Bâtiment 630, F-91405 Orsay cedex, France (C.H.-C.,
F.A.-B., R.R., C.C.d.F.-S.); and Laboratoire de Physiologie Cellulaire
et Moléculaire des Plantes, Centre National de la Recherche
Scientifique-Unité Mixte de Recherche 7632, 2135 Université Pierre et Marie Curie, Tour 53, case 154, 4 Place Jussieu, F-75252 Paris cedex 05, France (F.M.,
J.D.d.V.)
In higher plants formate
dehydrogenase (FDH, EC 1.2.1.2.) is a mitochondrial, NAD-dependent
enzyme. We previously reported that in potato (Solanum
tuberosum L.) FDH expression is high in tubers but low in green
leaves. Here we show that in isolated tuber mitochondria FDH is
involved in formate-dependent O2 uptake coupled to ATP
synthesis. The effects of various environmental and chemical factors on
FDH expression in leaves were tested using the mitochondrial serine
hydroxymethyltransferase as a control. The abundance of FDH transcripts
is strongly increased under various stresses, whereas serine
hydroxymethyltransferase transcripts decline. The application of
formate to leaves strongly enhances FDH expression, suggesting that it
might be the signal for FDH induction. Our experiments using glycolytic
products suggest that glycolysis may play an important role in formate
synthesis in leaves in the dark and during hypoxia, and in tubers. Of
particular interest is the dramatic accumulation of FDH transcripts
after spraying methanol on leaves, as this compound is known to
increase the yields of C3 plants. In addition, although the
steady-state levels of FDH transcript increase very quickly in response
to stress, protein accumulation is much slower, but can eventually reach the same levels in leaves as in tubers.
1
This work was supported by the Centre National
de la Recherche Scientifique (Strasbourg, France).
*
Corresponding author; e-mail colas{at}ibp.u-psud.fr; fax
33-169-33-63-24.
Plant Physiol. (1998) 116: 627-635
Copyright Clearance Center: 0032-0889/98/116/0627/09
© 1998 American Society of Plant Physiologists
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