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Induction and Regulation of Expression of a
Low-CO2-Induced Mitochondrial Carbonic Anhydrase in
Chlamydomonas reinhardtii
Mats Eriksson1, *,
Per Villand,
Per Gardeström, and
Göran Samuelsson
Department of Plant Physiology, Umeå University, S-901 87 Umeå,
Sweden
The time course of and the influence
of light intensity and light quality on the induction of a
mitochondrial carbonic anhydrase (CA) in the unicellular green alga
Chlamydomonas reinhardtii was characterized using
western and northern blots. This CA was expressed only under
low-CO2 conditions (ambient air). In asynchronously grown
cells, the mRNA was detected 15 min after transfer from air containing
5% CO2 to ambient air, and the 21-kD polypeptide was
detected on western blots after 1 h. When transferred back to air
containing 5% CO2, the mRNA disappeared within 1 h
and the polypeptide was degraded within 3 d. Photosynthesis was
required for the induction in asynchronous cultures. The induction
increased with light up to 500 µmol m 2
s1, where saturation occurred. In cells grown
synchronously, however, expression of the mitochondrial CA was also
detected in darkness. Under such conditions the expression followed a
circadian rhythm, with mRNA appearing in the dark 30 min before the
light was turned on. Algae left in darkness continued this rhythm for
several days.
1
Present address: Department of Chemistry and
Biochemistry, University of California, 405 Hilgard Avenue, Los
Angeles, CA 90095-1569.
*
Corresponding author; e-mail
mats.eriksson{at}plantphys.umu.se; fax 46-90-786-6676.
Plant Physiol. (1998) 116: 637-641
Copyright Clearance Center: 0032-0889/98/116/0637/05
© 1998 American Society of Plant Physiologists
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