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Re-Aeration following Hypoxia or Anoxia Leads to Activation
of the Antioxidative Defense System in Roots of Wheat
Seedlings1
Sophia Biemelt*,
Ulrich Keetman, and
Gerd Albrecht
Humboldt-Universität zu Berlin, Institut für Biologie,
Botanik und Biologiedidaktik, Späthstrasse 80/81, D-12437 Berlin,
Germany (S.B., G.A.); and Institut für Pflanzengenetik und
Kulturpflanzenforschung Gatersleben, Correnstrasse 3, D-06466
Gatersleben, Germany (U.K.)
The response of the
ascorbate-glutathione cycle was investigated in roots of young wheat
(Triticum aestivum L.) seedlings that were deprived of
oxygen either by subjecting them to root hypoxia or to entire plant
anoxia and then re-aerated. Although higher total levels of ascorbate
and glutathione were observed under hypoxia, only the total amount of
ascorbate was increased under anoxia. Under both treatments a
significant increase in the reduced form of ascorbate and glutathione
was found, resulting in increased reduction states. Upon the onset of
re-aeration the ratios started to decline rapidly, indicating oxidative
stress. Hypoxia caused higher activity of ascorbate peroxidase, whereas activities of monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were diminished or only slightly influenced. Under anoxia, activities of ascorbate peroxidase and glutathione reductase decreased significantly to 39 and 62%,
respectively. However, after re-aeration of hypoxically or anoxically
pretreated roots, activity of enzymes approached the control levels.
This corresponds with the restoration of the high reduction state of ascorbate and glutathione within 16 to 96 h of re-aeration,
depending on the previous duration of anoxia. Apparently, anoxia
followed by re-aeration more severely impairs entire plant metabolism
compared with hypoxia, thus leading to decreased viability.
1
This work was supported by a grant (Nafög)
to S.B. from the state of Berlin.
*
Corresponding author; e-mail h0567axw{at}rz.hu-berlin.de; fax
49-30-636-9446.
Plant Physiol. (1998) 116: 651-658
Copyright Clearance Center: 0032-0889/98/116/0651/08
© 1998 American Society of Plant Physiologists
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