Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (66)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seo, M.
Right arrow Articles by Koshiba, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seo, M.
Right arrow Articles by Koshiba, T.
Agricola
Right arrow Articles by Seo, M.
Right arrow Articles by Koshiba, T.

Higher Activity of an Aldehyde Oxidase in the Auxin-Overproducing superroot1 Mutant of Arabidopsis thaliana1

Mitsunori Seo, Shuichi Akaba, Takayuki Oritani, Marianne Delarue2, Catherine Bellini, Michel Caboche, and Tomokazu Koshiba*

Department of Biology, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan (M.S., S.A., T.K.); Department of Applied Biological Chemistry, Tohoku University, Aoba-ku, Sendai 981, Japan (T.O.); and Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles cedex, France (M.D., C.B., M.C.)

Aldehyde oxidase (AO; EC 1.2.3.1) activity was measured in seedlings of wild type or an auxin-overproducing mutant, superroot1 (sur1), of Arabidopsis thaliana. Activity staining for AO after native polyacrylamide gel electrophoresis separation of seedling extracts revealed that there were three major bands with AO activity (AO1-3) in wild-type and mutant seedlings. One of them (AO1) had a higher substrate preference for indole-3-aldehyde. This AO activity was significantly higher in sur1 mutant seedlings than in the wild type. The difference in activity was most apparent 7 d after germination, the same time required for the appearance of the remarkable sur1 phenotype, which includes epinastic cotyledons, elongated hypocotyls, and enhanced root development. Higher activity was observed in the root and hypocotyl region of the mutant seedlings. We also assayed the indole-3-acetaldehyde oxidase activity in extracts by high-performance liquid chromatography detection of indole-3-acetic acid (IAA). The activity was about 5 times higher in the extract of the sur1 seedlings, indicating that AO1 also has a substrate preference for abscisic aldehyde. Treatment of the wild-type seedlings with picloram or IAA caused no significant increase in AO1 activity. This result suggested that the higher activity of AO1 in sur1 mutant seedlings was not induced by IAA accumulation and, thus, strongly supports the possible role of AO1 in IAA biosynthesis in Arabidopsis seedlings.

1   This research was supported in part by a Grant-in-Aid for Japan-France Joint Study on Development in Higher Plants from the Ministry of Education, Science, Sports, and Culture, Japan.
2   Present address; Laboratoire de Biologie et Physiologie Vegetale, Faculte des Sciences, Universite d'Angers 2 Boulevard Lavoisier 49045 Angers cedex 01, France.
*   Corresponding author; e-mail koshiba-tomokazu{at}c.metro-u.ac.jp; fax 81-426-77-2559.

Plant Physiol. (1998) 116: 687-693
Copyright Clearance Center:   0032-0889/98/116/0687/07
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Ibdah, Y.-T. Chen, C. G. Wilkerson, and E. Pichersky
An Aldehyde Oxidase in Developing Seeds of Arabidopsis Converts Benzaldehyde to Benzoic Acid
Plant Physiology, May 1, 2009; 150(1): 416 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. A. Contreras-Cornejo, L. Macias-Rodriguez, C. Cortes-Penagos, and J. Lopez-Bucio
Trichoderma virens, a Plant Beneficial Fungus, Enhances Biomass Production and Promotes Lateral Root Growth through an Auxin-Dependent Mechanism in Arabidopsis
Plant Physiology, March 1, 2009; 149(3): 1579 - 1592.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Hirano, K. Aya, T. Hobo, H. Sakakibara, M. Kojima, R. A. Shim, Y. Hasegawa, M. Ueguchi-Tanaka, and M. Matsuoka
Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in Microspore/Pollen and Tapetum of Rice
Plant Cell Physiol., October 1, 2008; 49(10): 1429 - 1450.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Naponelli, A. Noiriel, M. J. Ziemak, S. M. Beverley, L.-F. Lye, A. M. Plume, J. R. Botella, K. Loizeau, S. Ravanel, F. Rebeille, et al.
Phylogenomic and Functional Analysis of Pterin-4a-Carbinolamine Dehydratase Family (COG2154) Proteins in Plants and Microorganisms
Plant Physiology, April 1, 2008; 146(4): 1515 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Ding, Y. Cao, L. Huang, J. Zhao, C. Xu, X. Li, and S. Wang
Activation of the Indole-3-Acetic Acid-Amido Synthetase GH3-8 Suppresses Expansin Expression and Promotes Salicylate- and Jasmonate-Independent Basal Immunity in Rice
PLANT CELL, January 1, 2008; 20(1): 228 - 240.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kaminaga, J. Schnepp, G. Peel, C. M. Kish, G. Ben-Nissan, D. Weiss, I. Orlova, O. Lavie, D. Rhodes, K. Wood, et al.
Plant Phenylacetaldehyde Synthase Is a Bifunctional Homotetrameric Enzyme That Catalyzes Phenylalanine Decarboxylation and Oxidation
J. Biol. Chem., August 18, 2006; 281(33): 23357 - 23366.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. W. WOODWARD and B. BARTEL
Auxin: Regulation, Action, and Interaction
Ann. Bot., April 1, 2005; 95(5): 707 - 735.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Seo, H. Aoki, H. Koiwai, Y. Kamiya, E. Nambara, and T. Koshiba
Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds
Plant Cell Physiol., November 15, 2004; 45(11): 1694 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Zdunek-Zastocka, R. T. Omarov, T. Koshiba, and H. S. Lips
Activity and protein level of AO isoforms in pea plants (Pisum sativum L.) during vegetative development and in response to stress conditions
J. Exp. Bot., June 1, 2004; 55(401): 1361 - 1369.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Gonzalez-Guzman, D. Abia, J. Salinas, R. Serrano, and P. L. Rodriguez
Two New Alleles of the abscisic aldehyde oxidase 3 Gene Reveal Its Role in Abscisic Acid Biosynthesis in Seeds
Plant Physiology, May 1, 2004; 135(1): 325 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. B. Nair, K. L. Bastress, M. O. Ruegger, J. W. Denault, and C. Chapple
The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 Gene Encodes an Aldehyde Dehydrogenase Involved in Ferulic Acid and Sinapic Acid Biosynthesis
PLANT CELL, February 1, 2004; 16(2): 544 - 554.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. J. Park, V. Kriechbaumer, A. Muller, M. Piotrowski, R. B. Meeley, A. Gierl, and E. Glawischnig
The Nitrilase ZmNIT2 Converts Indole-3-Acetonitrile to Indole-3-Acetic Acid
Plant Physiology, October 1, 2003; 133(2): 794 - 802.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. R. Jones, T. Manabe, M. Awazuhara, and K. Saito
A New Member of Plant CS-lyases. A CYSTINE LYASE FROM ARABIDOPSIS THALIANA
J. Biol. Chem., March 14, 2003; 278(12): 10291 - 10296.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. E. Davidson, R. C. Elliott, C. A. Helliwell, A. T. Poole, and J. B. Reid
The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase
Plant Physiology, January 1, 2003; 131(1): 335 - 344.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Liu and P. S. Schnable
Functional Specialization of Maize Mitochondrial Aldehyde Dehydrogenases
Plant Physiology, December 1, 2002; 130(4): 1657 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. L. Hale, S. P. McGrath, E. Lombi, S. M. Stack, N. Terry, I. J. Pickering, G. N. George, and E. A.H. Pilon-Smits
Molybdenum Sequestration in Brassica Species. A Role for Anthocyanins?
Plant Physiology, August 1, 2001; 126(4): 1391 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
New Insights into Auxin Biosynthesis
PLANT CELL, January 1, 2001; 13(1): 1 - 3.
[Full Text]

Home page
 Plant CellHome page
S. Bak, F. E. Tax, K. A. Feldmann, D. W. Galbraith, and R. Feyereisen
CYP83B1, a Cytochrome P450 at the Metabolic Branch Point in Auxin and Indole Glucosinolate Biosynthesis in Arabidopsis
PLANT CELL, January 1, 2001; 13(1): 101 - 111.
[Abstract] [Full Text]

Home page
 Plant CellHome page
G. Schwarz, J. Schulze, F. Bittner, T. Eilers, J. Kuper, G. Bollmann, A. Nerlich, H. Brinkmann, and R. R. Mendel
The Molybdenum Cofactor Biosynthetic Protein Cnx1 Complements Molybdate-Repairable Mutants, Transfers Molybdenum to the Metal Binding Pterin, and Is Associated with the Cytoskeleton
PLANT CELL, December 1, 2000; 12(12): 2455 - 2472.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Seo, A. J. M. Peeters, H. Koiwai, T. Oritani, A. Marion-Poll, J. A. D. Zeevaart, M. Koornneef, Y. Kamiya, and T. Koshiba
The Arabidopsis aldehyde oxidase 3 (AAO3) gene product catalyzes the final step in abscisic acid biosynthesis in leaves
PNAS, October 23, 2000; (2000) 220426197.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Kuper, T. Palmer, R. R. Mendel, and G. Schwarz
Mutations in the molybdenum cofactor biosynthetic protein Cnx1G from Arabidopsis thaliana define functions for molybdopterin binding, molybdenum insertion, and molybdenum cofactor stabilization
PNAS, June 6, 2000; 97(12): 6475 - 6480.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Seo, A. J. M. Peeters, H. Koiwai, T. Oritani, A. Marion-Poll, J. A. D. Zeevaart, M. Koornneef, Y. Kamiya, and T. Koshiba
The Arabidopsis aldehyde oxidase 3 (AAO3) gene product catalyzes the final step in abscisic acid biosynthesis in leaves
PNAS, November 7, 2000; 97(23): 12908 - 12913.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists