Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (64)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, S.-H.
Right arrow Articles by Seemann, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, S.-H.
Right arrow Articles by Seemann, J. R.
Agricola
Right arrow Articles by Cheng, S.-H.
Right arrow Articles by Seemann, J. R.

Effects of Short- and Long-Term Elevated CO2 on the Expression of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Genes and Carbohydrate Accumulation in Leaves of Arabidopsis thaliana (L.) Heynh.1

Shu-Hua Cheng*, Brandon d. Moore, and Jeffrey R. Seemann

Department of Biochemistry, University of Nevada, Reno, Nevada 89557

To investigate the proposed molecular characteristics of sugar-mediated repression of photosynthetic genes during plant acclimation to elevated CO2, we examined the relationship between the accumulation and metabolism of nonstructural carbohydrates and changes in ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) gene expression in leaves of Arabidopsis thaliana exposed to elevated CO2. Long-term growth of Arabidopsis at high CO2 (1000 µL L-1) resulted in a 2-fold increase in nonstructural carbohydrates, a large decrease in the expression of Rubisco protein and in the transcript of rbcL, the gene encoding the large subunit of Rubisco (approximately 35-40%), and an even greater decline in mRNA of rbcS, the gene encoding the small subunit (approximately 60%). This differential response of protein and mRNAs suggests that transcriptional/posttranscriptional processes and protein turnover may determine the final amount of leaf Rubisco protein at high CO2. Analysis of mRNA levels of individual rbcS genes indicated that reduction in total rbcS transcripts was caused by decreased expression of all four rbcS genes. Short-term transfer of Arabidopsis plants grown at ambient CO2 to high CO2 resulted in a decrease in total rbcS mRNA by d 6, whereas Rubisco content and rbcL mRNA decreased by d 9. Transfer to high CO2 reduced the maximum expression level of the primary rbcS genes (1A and, particularly, 3B) by limiting their normal pattern of accumulation through the night period. The decreased nighttime levels of rbcS mRNA were associated with a nocturnal increase in leaf hexoses. We suggest that prolonged nighttime hexose metabolism resulting from exposure to elevated CO2 affects rbcS transcript accumulation and, ultimately, the level of Rubisco protein.

1   This work was supported by National Science Foundation grant no. 1940424 to J.R.S. and S.-H.C.
*   Corresponding author; e-mail scheng{at}med.unr.edu; fax 1-702-784-1650.

Plant Physiol. (1998) 116: 715-723
Copyright Clearance Center:   0032-0889/98/116/0715/09
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Luo, H. Wei, L. Ye, K. Wang, F. Chen, L. Luo, L. Liu, Y. Li, M. J. C. Crabbe, L. Jin, et al.
Photosynthetic metabolism of C3 plants shows highly cooperative regulation under changing environments: A systems biological analysis
PNAS, January 20, 2009; 106(3): 847 - 852.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Patel and J. O. Berry
Rubisco gene expression in C4 plants
J. Exp. Bot., May 1, 2008; 59(7): 1625 - 1634.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Tholen, T. L. Pons, L. A.C.J. Voesenek, and H. Poorter
Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco
Plant Physiology, July 1, 2007; 144(3): 1305 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
J. A. Miklos, M. F. Alibhai, S. A. Bledig, D. C. Connor-Ward, A.-G. Gao, B. A. Holmes, K. H. Kolacz, V. T. Kabuye, T. C. MacRae, M. S. Paradise, et al.
Characterization of Soybean Exhibiting High Expression of a Synthetic Bacillus thuringiensis cry1A Transgene That Confers a High Degree of Resistance to Lepidopteran Pests
Crop Sci., January 22, 2007; 47(1): 148 - 157.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Patel, A. J. Siegel, and J. O. Berry
Untranslated Regions of FbRbcS1 mRNA Mediate Bundle Sheath Cell-specific Gene Expression in Leaves of a C4 Plant
J. Biol. Chem., September 1, 2006; 281(35): 25485 - 25491.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Araya, K. Noguchi, and I. Terashima
Effects of Carbohydrate Accumulation on Photosynthesis Differ between Sink and Source Leaves of Phaseolus vulgaris L.
Plant Cell Physiol., May 1, 2006; 47(5): 644 - 652.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. C. de Groot, R. van den Boogaard, L. F. M. Marcelis, J. Harbinson, and H. Lambers
Contrasting effects of N and P deprivation on the regulation of photosynthesis in tomato plants in relation to feedback limitation
J. Exp. Bot., August 1, 2003; 54(389): 1957 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Paul and T. K. Pellny
Carbon metabolite feedback regulation of leaf photosynthesis and development
J. Exp. Bot., January 3, 2003; 54(382): 539 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Sun, K. M. Gibson, O. Kiirats, T. W. Okita, and G. E. Edwards
Interactions of Nitrate and CO2 Enrichment on Growth, Carbohydrates, and Rubisco in Arabidopsis Starch Mutants. Significance of Starch and Hexose
Plant Physiology, November 1, 2002; 130(3): 1573 - 1583.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Onizuka, H. Akiyama, S. Endo, S. Kanai, M. Hirano, S. Tanaka, and H. Miyasaka
CO2 Response Element and Corresponding trans-acting Factor of the Promoter for Ribulose-1,5-bisphosphate Carboxylase/oxygenase Genes in Synechococcus sp. PCC7002 Found by an Improved Electrophoretic Mobility Shift Assay
Plant Cell Physiol., June 15, 2002; 43(6): 660 - 667.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. A. Lake, F. I. Woodward, and W. P. Quick
Long-distance CO2 signalling in plants
J. Exp. Bot., February 1, 2002; 53(367): 183 - 193.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Paul and C. H. Foyer
Sink regulation of photosynthesis
J. Exp. Bot., July 1, 2001; 52(360): 1383 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
D. Jenkins Klus, S. Kalisz, P. S. Curtis, J. A. Teeri, and S. J. Tonsor
Family- and population-level responses to atmospheric CO2 concentration: gas exchange and the allocation of C, N, and biomass in Plantago lanceolata (Plantaginaceae)
Am. J. Botany, June 1, 2001; 88(6): 1080 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. W. Gesch, K. J. Boote, J. C.V. Vu, L. Hartwell Allen Jr, and G. Bowes
Changes in Growth CO2 Result in Rapid Adjustments of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Small Subunit Gene Expression in Expanding and Mature Leaves of Rice
Plant Physiology, October 1, 1998; 118(2): 521 - 529.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists