Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ritchie, S.
Right arrow Articles by Gilroy, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ritchie, S.
Right arrow Articles by Gilroy, S.
Agricola
Right arrow Articles by Ritchie, S.
Right arrow Articles by Gilroy, S.

Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone1

Sian Ritchie and Simon Gilroy*

Biology Department, The Pennsylvania State University, 208 Mueller Laboratory, University Park, Pennsylvania 16802

Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+- and calmodulin (CaM)-dependent kinases, selectively inhibited the GA response, leaving constitutive and abscisic acid-regulated events unaffected. Microinjection of syntide did not affect the GA-induced increase in cytosolic [Ca2+], suggesting that it inhibited GA action downstream of the Ca2+ signal. When photoaffinity-labeled syntide-2 was electroporated into protoplasts and cross-linked to interacting proteins in situ, it selectively labeled proteins of approximately 30 and 55 kD. A 54-kD, soluble syntide-2 phosphorylating protein kinase was detected in aleurone cells. This kinase was activated by Ca2+ and was CaM independent, but was inhibited by the CaM antagonist N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide (250 µm), suggesting that it was a CaM-domain protein kinase-like activity. These results suggest that syntide-2 inhibits the GA response of the aleurone via an interaction with this kinase, implicating the 54-kD kinase as a Ca2+-dependent regulator of the GA response in these cells.

1   This work was supported by U.S. Department of Agriculture grant no. 94-37304-0955. The confocal microscope was supported by U.S. Department of Energy equipment grant no. DE-FG05-93ER79239.
*   Corresponding author; e-mail sxg12{at}psu.edu; fax 1-814-865-9131.

Plant Physiol. (1998) 116: 765-776
Copyright Clearance Center:   0032-0889/98/116/0765/12
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant CellHome page
G. M. Yoon, P. E. Dowd, S. Gilroy, and A. G. McCubbin
Calcium-Dependent Protein Kinase Isoforms in Petunia Have Distinct Functions in Pollen Tube Growth, Including Regulating Polarity
PLANT CELL, April 1, 2006; 18(4): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-s. Hwang, P. C. Bethke, Y. H. Cheong, H.-S. Chang, T. Zhu, and R. L. Jones
A Gibberellin-Regulated Calcineurin B in Rice Localizes to the Tonoplast and Is Implicated in Vacuole Function
Plant Physiology, July 1, 2005; 138(3): 1347 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. K. Azad, Y. Sawa, T. Ishikawa, and H. Shibata
Phosphorylation of Plasma Membrane Aquaporin Regulates Temperature-Dependent Opening of Tulip Petals
Plant Cell Physiol., May 1, 2004; 45(5): 608 - 617.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. C. Chang, M. H. Cho, S.-K. Kim, J. S. Lee, A. Kirakosyan, and P. B. Kaufman
Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots
J. Exp. Bot., March 1, 2003; 54(384): 1013 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Martín and L. Busconi
A Rice Membrane-Bound Calcium-Dependent Protein Kinase Is Activated in Response to Low Temperature
Plant Physiology, March 1, 2001; 125(3): 1442 - 1449.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
V. S. Anil, A. C. Harmon, and K. S. Rao
Spatio-Temporal Accumulation and Activity of Calcium-Dependent Protein Kinases during Embryogenesis, Seed Development, and Germination in Sandalwood
Plant Physiology, April 1, 2000; 122(4): 1035 - 1044.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
E. van der Knaap, W.-Y. Song, D.-L. Ruan, M. Sauter, P. C. Ronald, and H. Kende
Expression of a Gibberellin-Induced Leucine-Rich Repeat Receptor-Like Protein Kinase in Deepwater Rice and Its Interaction with Kinase-Associated Protein Phosphatase
Plant Physiology, June 1, 1999; 120(2): 559 - 570.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. Moutinho, A. J. Trewavas, and R. Malhó
Relocation of a Ca2+-Dependent Protein Kinase Activity during Pollen Tube Reorientation
PLANT CELL, September 1, 1998; 10(9): 1499 - 1510.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. X. Lu and E. M. Hrabak
An Arabidopsis Calcium-Dependent Protein Kinase Is Associated with the Endoplasmic Reticulum
Plant Physiology, March 1, 2002; 128(3): 1008 - 1021.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists