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Differential Expression of a Novel Gene in Response to
Coronatine, Methyl Jasmonate, and Wounding in the Coi1
Mutant of Arabidopsis1
Celso E. Benedetti*,
Cinthia L. Costa,
Silvia R. Turcinelli, and
Paulo Arruda
Centro de Biologia Molecular e Engenharia
Genética-Universidade Estadual de Campinas, Campinas,
São Paulo 13083-970, Brazil (C.E.B., C.L.C., S.R.T., P.A.); and Departamento de Genética e Evolução, Instituto
de Biologia, Universidade Estadual de Campinas, 13083-970, Campinas,
São Paulo, Brazil (P.A.)
Coronatine is a phytotoxin produced
by some plant-pathogenic bacteria. It has been shown that coronatine
mimics the action of methyl jasmonate (MeJA) in plants. MeJA is a
plant-signaling molecule involved in stress responses such as wounding
and pathogen attack. In Arabidopsis thaliana, MeJA is
essential for pollen grain development. The coi1 (for
coronatine-insensitive) mutant of Arabidopsis,
which is insensitive to coronatine and MeJA, produces sterile male
flowers and shows an altered response to wounding. When the
differential display technique was used, a message that was rapidly
induced by coronatine in wild-type plants but not in
coi1 was identified and the corresponding cDNA was
cloned. The coronatine-induced gene ATHCOR1 (for
A. thaliana coronatine-induced) is expressed in seedlings, mature
leaves, flowers, and siliques but was not detected in roots. The
expression of this gene was dramatically reduced in coi1
plants, indicating that COI1 affects its expression.
ATHCOR1 was rapidly induced by MeJA and wounding in
wild-type plants. The sequence of ATHCOR1 shows no
strong homology to known proteins. However, the predicted polypeptide
contains a conserved amino acid sequence present in several bacterial,
animal, and plant hydrolases and includes a potential
ATP/GTP-binding-site motif (P-loop).
1
This work was supported by grant no. 95/6662-5
from Fundação de Amparo à Pesquisa do Estado de
São Paulo. C.E.B. received a fellowship from Conselho Nacional de
Desenvolvimento Cientifico e Tecnológico (no. 300764/95-2) and
Fundação de Amparo à Pesquisa do Estado de São
Paulo (no. 97/0917-7). C.L.C. received a fellowship from
Fundação de Amparo à Pesquisa do Estado de São
Paulo (96/10274-3).
*
Corresponding author; e-mail benedett{at}turing.unicamp.br; fax
55-19-788-2193.
Plant Physiol. (1998) 116: 1037-1042
Copyright Clearance Center: 0032-0889/98/116/1037/06
© 1998 American Society of Plant Physiologists
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