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Heat-Stress Response of Maize Mitochondria1
Adrian A. Lund2,
Paul H. Blum,
Dinakar Bhattramakki3, and
Thomas E. Elthon*
School of Biological Sciences and the Center for Biotechnology,
University of Nebraska, Lincoln, Nebraska 68588-0118
We have
identified maize (Zea mays L. inbred B73) mitochondrial
homologs of the Escherichia coli molecular chaperones
DnaK (HSP70) and GroEL (cpn60) using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. During heat
stress (42°C for 4 h), levels of HSP70 and cpn60 proteins did
not change significantly. In contrast, levels of two 22-kD proteins
increased dramatically (HSP22). Monoclonal antibodies were developed to
maize HSP70, cpn60, and HSP22. The monoclonal antibodies were
characterized with regard to their cross-reactivity to chloroplastic,
cytosolic, and mitochondrial fractions, and to different plant species.
Expression of mitochondrial HSP22 was evaluated with regard to
induction temperature, time required for induction, and time required
for degradation upon relief of stress. Maximal HSP22 expression
occurred in etiolated seedling mitochondria after 5 h of a +13°C
heat stress. Upon relief of heat stress, the HSP22 proteins disappeared
with a half-life of about 4 h and were undetectable after 21 h of recovery. Under continuous heat-stress conditions, the level of
HSP22 remained high. A cDNA for maize mitochondrial HSP22 was cloned
and extended to full length with sequences from an expressed sequence
tag database. Sequence analysis indicated that HSP22 is a member of the
plant small heat-shock protein superfamily.
1
This work was supported in part by grants from
Pioneer Hi-Bred International, Inc., National Science
Foundation-Experimental Program to Stimulate Competitive Research
(EPS-9255225), and the Center for Biotechnology, University of
Nebraska-Lincoln.
2
Present address: Nebraska Center for Mass
Spectrometry, Department of Chemistry, University of Nebraska, Lincoln,
NE 68588-0304.
3
Present address: Crop Biotechnology Center,
Texas A&M University, College Station, TX 77843-2123.
*
Corresponding author; e-mail telthon{at}unl.edu; fax
1-402-472-2083.
Plant Physiol. (1998) 116: 1097-1110
Copyright Clearance Center: 0032-0889/98/116/1097/14
© 1998 American Society of Plant Physiologists
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