Mortal: A Mutant of White Clover Defective in Nodal Root Development

Derek W.R. White^*, Derek R. Woodfield, and John R. Caradus

Plant Molecular Genetics Laboratory (D.W.R.W.), and Plant Improvement Group (D.R.W., J.R.C.), Grasslands Division, AgResearch, Private Bag 11008, Palmerston North, New Zealand

A monogenic dominant mutant of white clover (Trifolium repens L.), designated Mortal, which is defective in the formation of adventitious nodal roots, is described. Mortal plants grown at temperatures ranging from 10 to 25°C do not initiate nodal root primordium development. However, all other aspects of plant development are normal, including the formation of lateral roots and wound-induced adventitious roots. In some genetic backgrounds, the Mortal mutation has a temperature-sensitive conditional phenotype. Mortal plants shifted from growing conditions of 20 to 30°C for 2 to 3 d form nodal root meristems. However, new nodes that develop after plants are returned to 20°C exhibit the mutant phenotype. The capacity to form nodal roots on cuttings placed in water is also influenced by the genetic background of the Mortal mutation. Genetic analysis established that the physiological reversion of Mortal to nodal root formation is controlled by at least two separate dominant genetic loci, one for Nodal water response (Now) and one for Nodal temperature response (Not); the Now locus has a dominant epistatic interaction with the Not locus. The conditional nature of Mortal should provide opportunities for the identification of genetic and physiological mechanisms that influence the development of nodal roots.

Plant Physiol. (1998) 116: 913-921
Copyright Clearance Center:   0032-0889/98/116/0913/09
© 1998 American Society of Plant Physiologists