Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (45)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patton, D. A.
Right arrow Articles by Meinke, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patton, D. A.
Right arrow Articles by Meinke, D. W.
Agricola
Right arrow Articles by Patton, D. A.
Right arrow Articles by Meinke, D. W.

An Embryo-Defective Mutant of Arabidopsis Disrupted in the Final Step of Biotin Synthesis

David A. Patton, Amy L. Schetter, Linda H. Franzmann, Karin Nelson, Eric R. Ward, and David W. Meinke*

Novartis Crop Protection, P.O. Box 12257, Research Triangle Park, North Carolina 27709 (D.A.P., K.N., E.R.W.); and Department of Botany, Oklahoma State University, Stillwater, Oklahoma 74078 (A.L.S., L.H.F., D.W.M.)

Auxotrophic mutants have played an important role in the genetic dissection of biosynthetic pathways in microorganisms. Equivalent mutants have been more difficult to identify in plants. The bio1 auxotroph of Arabidopsis thaliana was shown previously to be defective in the synthesis of the biotin precursor 7,8-diaminopelargonic acid. A second biotin auxotroph of A. thaliana has now been identified. Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Three lines of evidence indicate that bio2 is defective in biotin synthase production: mutant embryos are rescued by biotin but not dethiobiotin, the mutant allele maps to the same chromosomal location as the cloned biotin synthase gene, and gel-blot hybridizations and polymerase chain reaction amplifications revealed that homozygous mutant plants contain a deletion spanning the entire BIO2-coding region. Here we describe how the isolation and characterization of this null allele have provided valuable insights into biotin synthesis, auxotrophy, and gene redundancy in plants.

*   Corresponding author; e-mail meinke{at}osuunx.ucc.okstate.edu; fax 1-405-744-7074.

Plant Physiol. (1998) 116: 935-946
Copyright Clearance Center:   0032-0889/98/116/0935/12
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Chen, B. P. Mooney, M. Hajduch, T. Joshi, M. Zhou, D. Xu, and J. J. Thelen
System Analysis of an Arabidopsis Mutant Altered in de Novo Fatty Acid Synthesis Reveals Diverse Changes in Seed Composition and Metabolism
Plant Physiology, May 1, 2009; 150(1): 27 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Rajjou, Y. Lovigny, S. P.C. Groot, M. Belghazi, C. Job, and D. Job
Proteome-Wide Characterization of Seed Aging in Arabidopsis: A Comparison between Artificial and Natural Aging Protocols
Plant Physiology, September 1, 2008; 148(1): 620 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Muralla, E. Chen, C. Sweeney, J. A. Gray, A. Dickerman, B. J. Nikolau, and D. Meinke
A Bifunctional Locus (BIO3-BIO1) Required for Biotin Biosynthesis in Arabidopsis
Plant Physiology, January 1, 2008; 146(1): 60 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Muralla, C. Sweeney, A. Stepansky, T. Leustek, and D. Meinke
Genetic Dissection of Histidine Biosynthesis in Arabidopsis
Plant Physiology, June 1, 2007; 144(2): 890 - 903.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Tambasco-Studart, I. Tews, N. Amrhein, and T. B. Fitzpatrick
Functional Analysis of PDX2 from Arabidopsis, a Glutaminase Involved in Vitamin B6 Biosynthesis
Plant Physiology, June 1, 2007; 144(2): 915 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Pinon, S. Ravanel, R. Douce, and C. Alban
Biotin Synthesis in Plants. The First Committed Step of the Pathway Is Catalyzed by a Cytosolic 7-Keto-8-Aminopelargonic Acid Synthase
Plant Physiology, December 1, 2005; 139(4): 1666 - 1676.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Tambasco-Studart, O. Titiz, T. Raschle, G. Forster, N. Amrhein, and T. B. Fitzpatrick
Vitamin B6 biosynthesis in higher plants
PNAS, September 20, 2005; 102(38): 13687 - 13692.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Tzafrir, R. Pena-Muralla, A. Dickerman, M. Berg, R. Rogers, S. Hutchens, T. C. Sweeney, J. McElver, G. Aux, D. Patton, et al.
Identification of Genes Required for Embryo Development in Arabidopsis
Plant Physiology, July 1, 2004; 135(3): 1206 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. T. Song, H. Lu, and J. T. Greenberg
Divergent Roles in Arabidopsis thaliana Development and Defense of Two Homologous Genes, ABERRANT GROWTH AND DEATH2 and AGD2-LIKE DEFENSE RESPONSE PROTEIN1, Encoding Novel Aminotransferases
PLANT CELL, February 1, 2004; 16(2): 353 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Li, Y. Zhao, B. C. McCaig, B. A. Wingerd, J. Wang, M. E. Whalon, E. Pichersky, and G. A. Howe
The Tomato Homolog of CORONATINE-INSENSITIVE1 Is Required for the Maternal Control of Seed Maturation, Jasmonate-Signaled Defense Responses, and Glandular Trichome Development
PLANT CELL, January 1, 2004; 16(1): 126 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. A. Sparkes, F. Brandizzi, S. P. Slocombe, M. El-Shami, C. Hawes, and A. Baker
An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis
Plant Physiology, December 1, 2003; 133(4): 1809 - 1819.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
A. Picciocchi, R. Douce, and C. Alban
The Plant Biotin Synthase Reaction: IDENTIFICATION AND CHARACTERIZATION OF ESSENTIAL MITOCHONDRIAL ACCESSORY PROTEIN COMPONENTS
J. Biol. Chem., June 27, 2003; 278(27): 24966 - 24975.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Che, L. M. Weaver, E. S. Wurtele, and B. J. Nikolau
The Role of Biotin in Regulating 3-Methylcrotonyl-Coenzyme A Carboxylase Expression in Arabidopsis
Plant Physiology, March 1, 2003; 131(3): 1479 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. G. Stacey, S. Koh, J. Becker, and G. Stacey
AtOPT3, a Member of the Oligopeptide Transporter Family, Is Essential for Embryo Development in Arabidopsis
PLANT CELL, November 1, 2002; 14(11): 2799 - 2811.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. R. Apuya, R. Yadegari, R. L. Fischer, J. J. Harada, and R. B. Goldberg
RASPBERRY3 Gene Encodes a Novel Protein Important for Embryo Development
Plant Physiology, June 1, 2002; 129(2): 691 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Tzafrir, J. A. McElver, C.-m. Liu, L. J. Yang, J. Q. Wu, A. Martinez, D. A. Patton, and D. W. Meinke
Diversity of TITAN Functions in Arabidopsis Seed Development
Plant Physiology, January 1, 2002; 128(1): 38 - 51.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. McElver, I. Tzafrir, G. Aux, R. Rogers, C. Ashby, K. Smith, C. Thomas, A. Schetter, Q. Zhou, M. A. Cushman, et al.
Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana
Genetics, December 1, 2001; 159(4): 1751 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Picciocchi, R. Douce, and C. Alban
Biochemical Characterization of the Arabidopsis Biotin Synthase Reaction. The Importance of Mitochondria in Biotin Synthesis
Plant Physiology, November 1, 2001; 127(3): 1224 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. M. Schomburg, D. A. Patton, D. W. Meinke, and R. M. Amasino
FPA, a Gene Involved in Floral Induction in Arabidopsis, Encodes a Protein Containing RNA-Recognition Motifs
PLANT CELL, June 1, 2001; 13(6): 1427 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. R. Apuya, R. Yadegari, R. L. Fischer, J. J. Harada, J. L. Zimmerman, and R. B. Goldberg
The Arabidopsis Embryo Mutant schlepperless Has a Defect in the Chaperonin-60{alpha} Gene
Plant Physiology, June 1, 2001; 126(2): 717 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Gallardo, C. Job, S. P.C. Groot, M. Puype, H. Demol, J. Vandekerckhove, and D. Job
Proteomic Analysis of Arabidopsis Seed Germination and Priming
Plant Physiology, June 1, 2001; 126(2): 835 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. McElver, D. Patton, M. Rumbaugh, C.-m. Liu, L. J. Yang, and D. Meinke
The TITAN5 Gene of Arabidopsis Encodes a Protein Related to the ADP Ribosylation Factor Family of GTP Binding Proteins
PLANT CELL, August 1, 2000; 12(8): 1379 - 1392.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists