Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (57)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McKenzie, M. J.
Right arrow Articles by Jameson, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McKenzie, M. J.
Right arrow Articles by Jameson, P. E.
Agricola
Right arrow Articles by McKenzie, M. J.
Right arrow Articles by Jameson, P. E.

Controlled Cytokinin Production in Transgenic Tobacco Using a Copper-Inducible Promoter

Marian Jane McKenzie1, *, Vadim Mett, Paul Hugh Stewart Reynolds, and Paula Elizabeth Jameson

Botany Department, University of Otago, Private Bag 56, Dunedin, New Zealand (M.J.M.); Plant Improvement Division, The Horticulture and Food Research Institute of New Zealand, Private Bag 11030, Palmerston North, New Zealand (V.M., P.H.S.R.); and Department of Plant Biology and Biotechnology2, Massey University, Private Bag 11222, Palmerston North, New Zealand (P.E.J.)

The cytokinin group of plant hormones regulates aspects of plant growth and development, including the release of lateral buds from apical dominance and the delay of senescence. In this work the native promoter of a cytokinin synthase gene (ipt) was removed and replaced with a Cu-controllable promoter. Tobacco (Nicotiana tabacum L. cv tabacum) transformed with this Cu-inducible ipt gene (Cu-ipt) was morphologically identical to controls under noninductive conditions in almost all lines produced. However, three lines grew in an altered state, which is indicative of cytokinin overproduction and was confirmed by a full cytokinin analysis of one of these lines. The in vitro treatment of morphologically normal Cu-ipt transformants with Cu2+ resulted in delayed leaf senescence and an increase in cytokinin concentration in the one line analyzed. In vivo, inductive conditions resulted in a significant release of lateral buds from apical dominance. The morphological changes seen during these experiments may reflect the spatial aspect of control exerted by this gene expression system, namely expression from the root tissue only. These results confirmed that endogenous cytokinin concentrations in tobacco transformants can be temporally and spatially controlled by the induction of ipt gene expression through the Cu-controllable gene-expression system.

1   Present address: Department of Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North, New Zealand.
*   Corresponding author; e-mail mmckenzie{at}hort.cri.nz; fax 64-6-350-5694.

Plant Physiol. (1998) 116: 969-977
Copyright Clearance Center:   0032-0889/98/116/0969/09
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Hayward, P. Stirnberg, C. Beveridge, and O. Leyser
Interactions between Auxin and Strigolactone in Shoot Branching Control
Plant Physiology, September 1, 2009; 151(1): 400 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. M. Rivero, V. Shulaev, and E. Blumwald
Cytokinin-Dependent Photorespiration and the Protection of Photosynthesis during Water Deficit
Plant Physiology, July 1, 2009; 150(3): 1530 - 1540.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Dong, Y. Niu, W. Li, and D. Zhang
Effects of cotton rootstock on endogenous cytokinins and abscisic acid in xylem sap and leaves in relation to leaf senescence
J. Exp. Bot., April 1, 2008; 59(6): 1295 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Kuderova, I. Urbankova, M. Valkova, J. Malbeck, B. Brzobohaty, D. Nemethova, and J. Hejatko
Effects of Conditional IPT-Dependent Cytokinin Overproduction on Root Architecture of Arabidopsis Seedlings
Plant Cell Physiol., April 1, 2008; 49(4): 570 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Qi, Y. Zhang, and T. Chai
Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal Responsiveness
Plant Physiology, January 1, 2007; 143(1): 50 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-S. You, H. Marella, R. Zentella, Y. Zhou, T. Ulmasov, T.-H. D. Ho, and R. S. Quatrano
Use of bacterial quorum-sensing components to regulate gene expression in plants.
Plant Physiology, April 1, 2006; 140(4): 1205 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Aloni, M. Langhans, E. Aloni, E. Dreieicher, and C. I. Ullrich
Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream
J. Exp. Bot., June 1, 2005; 56(416): 1535 - 1544.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
M. X. Cao, J. Q. Huang, Z. M. Wei, Q. H. Yao, C. Z. Wan, and J. A. Lu
Engineering Higher Yield and Herbicide Resistance in Rice by Agrobacterium-Mediated Multiple Gene Transformation
Crop Sci., November 1, 2004; 44(6): 2206 - 2213.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. Tang and R. J. Newton
Regulated gene expression by glucocorticoids in cultured Virginia pine (Pinus virginiana Mill.) cells
J. Exp. Bot., July 1, 2004; 55(402): 1499 - 1508.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Brugiere, S. Jiao, S. Hantke, C. Zinselmeier, J. A. Roessler, X. Niu, R. J. Jones, and J. E. Habben
Cytokinin Oxidase Gene Expression in Maize Is Localized to the Vasculature, and Is Induced by Cytokinins, Abscisic Acid, and Abiotic Stress
Plant Physiology, July 1, 2003; 132(3): 1228 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. M. Rosin, J. K. Hart, H. Van Onckelen, and D. J. Hannapel
Suppression of a Vegetative MADS Box Gene of Potato Activates Axillary Meristem Development
Plant Physiology, April 1, 2003; 131(4): 1613 - 1622.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Guivarc'h, J. Rembur, M. Goetz, T. Roitsch, M. Noin, T. Schmulling, and D. Chriqui
Local expression of the ipt gene in transgenic tobacco (Nicotiana tabacum L. cv. SR1) axillary buds establishes a role for cytokinins in tuberization and sink formation
J. Exp. Bot., April 1, 2002; 53(369): 621 - 629.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Leon, J. Royo, G. Vancanneyt, C. Sanz, H. Silkowski, G. Griffiths, and J. J. Sanchez-Serrano
Lipoxygenase H1 Gene Silencing Reveals a Specific Role in Supplying Fatty Acid Hydroperoxides for Aliphatic Aldehyde Production
J. Biol. Chem., January 4, 2002; 277(1): 416 - 423.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
G. Frugis, D. Giannino, G. Mele, C. Nicolodi, A. Chiappetta, M. B. Bitonti, A. M. Innocenti, W. Dewitte, H. Van Onckelen, and D. Mariotti
Overexpression of KNAT1 in Lettuce Shifts Leaf Determinate Growth to a Shoot-Like Indeterminate Growth Associated with an Accumulation of Isopentenyl-Type Cytokinins
Plant Physiology, August 1, 2001; 126(4): 1370 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Haran, S. Logendra, M. Seskar, M. Bratanova, and I. Raskin
Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression
Plant Physiology, October 1, 2000; 124(2): 615 - 626.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists