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Induced beta -Carotene Synthesis Driven by Triacylglycerol Deposition in the Unicellular Alga Dunaliella bardawil1

Said Rabbani, Peter Beyer, Johannes v. Lintig, Philippe Hugueney, and Hans Kleinig*

Institut für Biologie II, Zellbiologie, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany

Under stress conditions such as high light intensity or nutrient starvation, cells of the unicellular alga Dunaliella bardawil overproduce beta -carotene, which is accumulated in the plastids in newly formed triacylglycerol droplets. We report here that the formation of these sequestering structures and beta -carotene are interdependent. When the synthesis of triacylglycerol is blocked, the overproduction of beta -carotene is also inhibited. During overproduction of beta -carotene no up-regulation of phytoene synthase or phytoene desaturase is observed on the transcriptional or translational level, whereas at the same time acetyl-CoA carboxylase, the key regulatory enzyme of acyl lipid biosynthesis, is increased, at least in its enzymatic activity. We conclude that under normal conditions the carotenogenic pathway is not maximally active and may be appreciably stimulated in the presence of sequestering structures, creating a plastid-localized sink for the end product of the carotenoid biosynthetic pathway.

1   This study was supported in part by the Deutscher Akademischer Austauschdienst (S.R.), by a European Molecular Biology Organization long-term fellowship (P.H.), by the German-Israeli Foundation (no. 214-240.12/91), and by the European Community BIOTECH Program.
*   Corresponding author; e-mail kleinig{at}sun1.ruf.uni-freiburg.de; fax 49-761-203-2675.

Plant Physiol. (1998) 116: 1239-1248
Copyright Clearance Center:   0032-0889/98/116/1239/10
© 1998 American Society of Plant Physiologists




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