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Expression and Localization of Phosphoenolpyruvate Carboxylase in Developing and Germinating Wheat Grains1

María-Cruz González, Lidia Osuna, Cristina Echevarría, Jean Vidal, and Francisco J. Cejudo*

Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas "Isla de la Cartuja," Avda Américo Vespucio s/n, 41092-Sevilla, Spain (M.-C.G., F.J.C.); Departamento de Biología Vegetal, Facultad de Biología, Universidad de Sevilla, Spain (L.O., C.E.); and Institut de Biotechnologie des Plantes, Unité, Recherche Associé, Centre National de la Recherche Scientifique D 1128, Bâtiment 630, Université de Paris-Sud, Centre d'Orsay cedex, France (J.V.)

Phosphoenolpyruvate carboxylase (PEPC) activity and corresponding mRNA levels were investigated in developing and germinating wheat (Triticum aestivum) grains. During grain development PEPC activity increased to reach a maximum 15 d postanthesis. Western-blot experiments detected two main PEPC polypeptides with apparent molecular masses of 108 and 103 kD. The most abundant 103-kD PEPC subunit remained almost constant throughout the process of grain development and in the scutellum and aleurone layer of germinating grains. The less-abundant 108-kD polypeptide progressively disappeared during the second half of grain development and was newly synthesized in the scutellum and aleurone layer of germinating grains. PEPC mRNA was detected throughout the process of grain development; however, in germinating grains PEPC mRNA accumulated transiently in the scutellum and aleurone layer, showing a sharp maximum 24 h after imbibition. Immunolocalization studies revealed the presence of the enzyme in tissues with a high metabolic activity, as well as in the vascular tissue of the crease area of developing grains. A clear increase in PEPC was observed in the scutellar epithelium of grains 24 h after imbibition. The data suggest that the transiently formed PEPC mRNA in the scutellar epithelium encodes the 108-kD PEPC subunit.

1   This work was supported by grant no. PB92-0675 from Dirección General de Investigación Científica y Técnica, Ministerio de Educación y Ciencia, and grant no. CVI 0118 from Junta de Andalucía, Spain.
*   Corresponding author; e-mail fjcejudo{at}cica.es; fax 34-5-446-0065.

Plant Physiol. (1998) 116: 1249-1258
Copyright Clearance Center:   0032-0889/98/116/1249/10
© 1998 American Society of Plant Physiologists




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