Plant Physiol. Drug Metab Dispos
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Biological Activity of Reducing-End-Derivatized Oligogalacturonides in Tobacco Tissue Cultures1

Mark D. Spiro2, 3, Brent L. Ridley3, Stefan Eberhard, Keith A. Kates, Yves Mathieu, Malcolm A. O'Neill, Debra Mohnen, Jean Guern, Alan Darvill, and Peter Albersheim*

Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 220 Riverbend Road, Athens, Georgia 30602-4712 (B.L.R., S.E., K.A.K., M.A.O., D.M., A.D., P.A., M.D.S.); and Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Bâtiment 22-23, Avenue de la Terrasse, F-91198 Gif-sur-Yvette cedex, France (Y.M., J.G.)

The biological activity of reducing-end-modified oligogalacturonides was quantified in four tobacco (Nicotiana tabacum) tissue culture bioassays. The derivatives used were oligogalacturonides with the C-1 of their reducing end (a) covalently linked to a biotin hydrazide, (b) covalently linked to tyramine, (c) chemically reduced to a primary alcohol, or (d) enzymatically oxidized to a carboxylic acid. These derivatives were tested for their ability to (a) alter morphogenesis of N. tabacum cv Samsun thin cell-layer explants, (b) elicit extracellular alkalinization by suspension-cultured cv Samsun cells, (c) elicit extracellular alkalinization by suspension-cultured N. tabacum cv Xanthi cells, and (d) elicit H2O2 accumulation in the cv Xanthi cells. In all four bioassays, each of the derivatives had reduced biological activity compared with the corresponding underivatized oligogalacturonides, demonstrating that the reducing end is a key element for the recognition of oligogalacturonides in these systems. However, the degree of reduction in biological activity depends on the tissue culture system used and on the nature of the specific reducing-end modification. These results suggest that oligogalacturonides are perceived differently in each tissue culture system.

1   This research was supported in part by the U.S. Department of Energy (DOE)-funded Center for Plant and Microbial Complex Carbohydrates (grant no. DE-FG05-93-ER20097) and by DOE grant no. DE-FG02-96ER20221 (to P.A.).
2   Present address: Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802.
3   Both of these authors contributed equally to this manuscript.
*   Corresponding author; e-mail palbersh{at}ccrc.uga.edu; fax 1-706-542-4412.

Plant Physiol. (1998) 116: 1289-1298
Copyright Clearance Center:   0032-0889/98/116/1289/10
© 1998 American Society of Plant Physiologists




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