Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hatzfeld, Y.
Right arrow Articles by Davidian, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hatzfeld, Y.
Right arrow Articles by Davidian, J.-C.
Agricola
Right arrow Articles by Hatzfeld, Y.
Right arrow Articles by Davidian, J.-C.

Effect of ATP Sulfurylase Overexpression in Bright Yellow 2 Tobacco Cells1
Regulation of ATP Sulfurylase and SO42- Transport Activities

Yves Hatzfeld, Nicole Cathala, Claude Grignon, and Jean-Claude Davidian*

Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, Ecole Nationale Supérieure Agronomique de Montpellier, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique (Unité de Recherche Associée No. 2133), 2 Place Pierre Viala, 34060 Montpellier cedex 1, France

To determine if the ATP sulfurylase reaction is a regulatory step for the SO42--assimilation pathway in plants, an Arabidopsis thaliana ATP sulfurylase cDNA, APS2, was fused to the 35S promoter of the cauliflower mosaic virus and introduced by Agrobacterium tumefaciens-mediated transformation into isolated Bright Yellow 2 tobacco (Nicotiana tabacum) cells. The ATP sulfurylase activity in transgenic cells was 8-fold that in control cells, and was correlated with the expression of a specific polypeptide revealed by western analysis using an anti-ATP sulfurylase antibody. The molecular mass of this polypeptide agreed with that for the overexpressed mature protein. ATP sulfurylase overexpression had no effect on [35S]SO42- influx or ATP sulfurylase activity regulation by S availability, except that ATP sulfurylase activity variations in response to S starvation in transgenic cells were 8 times higher than in the wild type. There were also no differences in cell growth or sensitivity to SeO42- (a toxic SO42- analog) between transgenic and wild-type cells. We propose that in Bright Yellow 2 tobacco cells, the ATP sulfurylase derepression by S deficiency may involve a posttranscriptional mechanism, and that the ATP sulfurylase abundance is not limiting for cell metabolism.

1   This work was supported by the Institut National de la Recherche Agronomique, the Centre National de la Recherche Scientifique, and the Ecole Nationale Supérieure Agronomique de Montpellier. Y.H. was supported by a doctoral grant (no. 94123) from the Direction Générale de l'Enseignement et de la Recherche, Ministère de l'Agriculture.
*   Corresponding author; e-mail davidian{at}ensam.inra.fr; fax 33-467-52-57-37.

Plant Physiol. (1998) 116: 1307-1313
Copyright Clearance Center:   0032-0889/98/116/1307/07
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. El Kassis, N. Cathala, H. Rouached, P. Fourcroy, P. Berthomieu, N. Terry, and J.-C. Davidian
Characterization of a Selenate-Resistant Arabidopsis Mutant. Root Growth as a Potential Target for Selenate Toxicity
Plant Physiology, March 1, 2007; 143(3): 1231 - 1241.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Meyer and M. D. Fricker
Control of Demand-Driven Biosynthesis of Glutathione in Green Arabidopsis Suspension Culture Cells
Plant Physiology, December 1, 2002; 130(4): 1927 - 1937.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Leustek and K. Saito
Sulfate Transport and Assimilation in Plants
Plant Physiology, July 1, 1999; 120(3): 637 - 644.
[Full Text]

Home page
 Plant Physiol.Home page
E. A.H. Pilon-Smits, S. Hwang, C. Mel Lytle, Y. Zhu, J. C. Tai, R. C. Bravo, Y. Chen, T. Leustek, and N. Terry
Overexpression of ATP Sulfurylase in Indian Mustard Leads to Increased Selenate Uptake, Reduction, and Tolerance
Plant Physiology, January 1, 1999; 119(1): 123 - 132.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists