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Cyst(e)ine Is the Transport Metabolite of Assimilated Sulfur from
Bundle-Sheath to Mesophyll Cells in Maize Leaves1
Marta Burgener,
Marianne Suter,
Stephanie Jones, and
Christian Brunold*
Institute of Plant Physiology, Altenbergrain 21, CH-3013 Berne,
Switzerland
The intercellular distribution of the
enzymes and metabolites of assimilatory sulfate reduction and
glutathione synthesis was analyzed in maize (Zea mays L. cv LG 9) leaves. Mesophyll cells and strands of bundle-sheath cells
from second leaves of 11-d-old maize seedlings were obtained by two
different mechanical-isolation methods. Cross-contamination of cell
preparations was determined using ribulose bisphosphate carboxylase (EC
4.1.1.39) and nitrate reductase (EC 1.6.6.1) as marker enzymes for
bundle-sheath and mesophyll cells, respectively. ATP sulfurylase (EC
2.7.7.4) and adenosine 5 -phosphosulfate sulfotransferase activities
were detected almost exclusively in the bundle-sheath cells, whereas
GSH synthetase (EC 6.3.2.3) and cyst(e)ine,  -glutamylcysteine, and
glutathione were located predominantly in the mesophyll cells. Feeding
experiments using [35S]sulfate with intact leaves
indicated that cyst(e)ine was the transport metabolite of reduced
sulfur from bundle-sheath to mesophyll cells. This result was
corroborated by tracer experiments, which showed that isolated
bundle-sheath strands fed with [35S]sulfate secreted
radioactive cyst(e)ine as the sole thiol into the resuspending medium.
The results presented in this paper show that assimilatory sulfate
reduction is restricted to the bundle-sheath cells, whereas the
formation of glutathione takes place predominantly in the mesophyll
cells, with cyst(e)ine functioning as a transport metabolite between
the two cell types.
1
This work was supported by the Swiss National
Science Foundation. The term "Cys" is used when it is clear that
cystine is not involved; "cyst(e)ine" is used for an undefined
mixture of Cys and cystine. The concentrations are expressed in all
cases relative to Cys.
*
Corresponding author; e-mail chbrunold{at}pfp.unibe.ch; fax
41-31-332-20-59.
Plant Physiol. (1998) 116: 1315-1322
Copyright Clearance Center: 0032-0889/98/116/1315/08
© 1998 American Society of Plant Physiologists
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