Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms¹

G. Paul Bolwell, Dewi R. Davies, Chris Gerrish, Chung-Kyoon Auh², and Terence M. Murphy^*

Division of Biochemistry, School of Biological Sciences, Royal Holloway, and Bedford New College, University of London, Egham, Surrey TW20 0EX, United Kingdom (G.P.B., D.R.D., C.G.); and Section of Plant Biology, Division of Biological Sciences, University of California, Davis, California 95616 (C.-K.A., T.M.M.)

Cultured cells of rose (Rosa damascena) treated with an elicitor derived from Phytophthora spp. and suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H₂O₂. It has been hypothesized that in rose cells H₂O₂ is produced by a plasma membrane NAD(P)H oxidase (superoxide synthase), whereas in bean cells H₂O₂ is derived directly from cell wall peroxidases following extracellular alkalinization and the appearance of a reductant. In the rose/Phytophthora spp. system treated with N,N-diethyldithiocarbamate, superoxide was detected by a N,N-dimethyl-9,9-biacridium dinitrate-dependent chemiluminescence; in contrast, in the bean/C. lindemuthianum system, no superoxide was detected, with or without N,N-diethyldithiocarbamate. When rose cells were washed free of medium (containing cell wall peroxidase) and then treated with Phytophthora spp. elicitor, they accumulated a higher maximum concentration of H₂O₂ than when treated without the washing procedure. In contrast, a washing treatment reduced the H₂O₂ accumulated by French bean cells treated with C. lindemuthianum elicitor. Rose cells produced reductant capable of stimulating horseradish (Armoracia lapathifolia) peroxidase to form H₂O₂ but did not have a peroxidase capable of forming H₂O₂ in the presence of reductant. Rose and French bean cells thus appear to be responding by different mechanisms to generate the oxidative burst.

Plant Physiol. (1998) 116: 1379-1385
Copyright Clearance Center:   0032-0889/98/116/1379/07
© 1998 American Society of Plant Physiologists

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