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Nuclear-Gene Mutations Suppress a Defect in the Expression of the Chloroplast-Encoded Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase1

Seokjoo Hong2 and Robert J. Spreitzer*

Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664

The green alga Chlamydomonas reinhardtii mutant 76-5EN lacks photosynthesis because of a nuclear-gene mutation that specifically inhibits expression of the chloroplast gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39). Photosynthesis-competent revertants were selected from mutant 76-5EN to explore the possibility of increasing Rubisco expression. Genetic analysis of 10 revertants revealed that most arose from suppressor mutations in nuclear genes distinct from the original 76-5EN mutant gene. The revertant strains have regained various levels of Rubisco holoenzyme, but none of the suppressor mutations increased Rubisco expression above the wild-type level in either the presence or absence of the 76-5EN mutation. One suppressor mutation, S107-4B, caused a temperature-conditional, photosynthesis-deficient phenotype in the absence of the original 76-5EN mutation. The S107-4B strain was unable to grow photosynthetically at 35°C, but it expressed a substantial level of Rubisco holoenzyme. Whereas the 76-5EN gene encodes a nuclear factor that appears to be required for the transcription of the Rubisco large-subunit gene, the S107-4B nuclear gene may be required for the expression of other chloroplast genes.

1   This work was supported by a U.S. Department of Agriculture/National Research Initiative Competitive Grant (no. 94-37306-0349) and published as paper no. 12,011 of the Nebraska Agricultural Research Division journal series.
2   Present address: Department of Biochemistry, Michigan State University, East Lansing, MI 48824.
*   Corresponding author; e-mail rjs{at}unlinfo.unl.edu; fax 1-402-472-7842.

Plant Physiol. (1998) 116: 1387-1392
Copyright Clearance Center:   0032-0889/98/116/1387/06
© 1998 American Society of Plant Physiologists




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P. A. Lefebvre and C. D. Silflow
Chlamydomonas: The Cell and Its Genomes
Genetics, January 1, 1999; 151(1): 9 - 14.
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