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Accumulation of a Clock-Regulated Transcript during Flower-Inductive Darkness in Pharbitis nil1

Kimiyo Sage-Ono*, Michiyuki Ono, Hiroshi Harada, and Hiroshi Kamada

Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan (K.S.O., H.H., H.K.); and Biotechnology Institute, Akita Prefectural College of Agriculture, Ohgata, Akita, 010-0444 Japan (M.O.)

To clarify the molecular basis of the photoperiodic induction of flowering in the short-day plant Pharbitis nil cv Violet, we examined changes in the level of mRNA in cotyledons during the flower-inductive photoperiod using the technique of differential display by the polymerase chain reaction. A transcript that accumulated during the inductive dark period was identified and a cDNA corresponding to the transcript, designated PnC401 (P. nil C401), was isolated. RNA-blot hybridization verified that levels of PnC401 mRNA fluctuated with a circadian rhythm, with maxima between 12 and 16 h after the beginning of the dark period) and minima of approximately 0. This oscillation continued even during an extended dark period but was damped under continuous light. Accumulation of PnC401 mRNA was reduced by a brief exposure to red light at the 8th h of the dark period (night-break treatment) or by exposure to far-red light at the end of the light period (end-of-day far-red treatment). These results suggest that fluctuations in levels of PnC401 mRNA are regulated by phytochrome(s) and a circadian clock and that they are associated with photoperiodic events that include induction of flowering.

1   This work was supported in part by a Grant-in-Aid for Special Research Areas (grant no. 07281103; Genetic Dissection of Sexual Differentiation and Pollination Processes in Higher Plants) from the Ministry of Education, Science, Culture, and Sports, Japan, and by a Grant-in-Aid for the "Research for the Future" Program (grant no. JSPS-RFTF97L00601) from the Japan Society for the Promotion of Science.
*   Corresponding author; e-mail kimiyo{at}sakura.cc.tsukuba.ac.jp; fax 81-298-53-6006.

Plant Physiol. (1998) 116: 1479-1485
Copyright Clearance Center:   0032-0889/98/116/1479/07
© 1998 American Society of Plant Physiologists




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