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Dim-Red-Light-Induced Increase in Polar Auxin Transport in Cucumber Seedlings1
I. Development of Altered Capacity, Velocity, and Response to Inhibitors

James R. Shinkle*, Rajan Kadakia, and Alan M. Jones

Department of Biology, Trinity University, San Antonio, Texas 78212-7200 (J.R.S., R.K.); and Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280 (A.M.J.)

We have developed and characterized a system to analyze light effects on auxin transport independent of photosynthetic effects. Polar transport of [3H]indole-3-acetic acid through hypocotyl segments from etiolated cucumber (Cucumis sativus L.) seedlings was increased in seedlings grown in dim-red light (DRL) (0.5 µmol m-2 s-1) relative to seedlings grown in darkness. Both transport velocity and transport intensity (export rate) were increased by at least a factor of 2. Tissue formed in DRL completely acquired the higher transport capacity within 50 h, but tissue already differentiated in darkness acquired only a partial increase in transport capacity within 50 h of DRL, indicating a developmental window for light induction of commitment to changes in auxin transport. This light-induced change probably manifests itself by alteration of function of the auxin efflux carrier, as revealed using specific transport inhibitors. Relative to dark controls, DRL-grown seedlings were differentially less sensitive to two inhibitors of polar auxin transport, N-(naphth-1-yl) phthalamic acid and 2,3,5-triiodobenzoic acid. On the basis of these data, we propose that the auxin efflux carrier is a key target of light regulation during photomorphogenesis.

1   This work was supported by the U.S. Department of Agriculture-National Research Inititiative Competitive Grants Program (grant no. 93-37311-9590), a Faculty Development grant from Trinity University to J.R.S., and an undergraduate research fellowship to R.K. from the Pew Charitable Trusts through the Pew Mid-States Consortium for Science and Mathematics.
*   Corresponding author; e-mail jshinkle{at}trinity.edu; fax 1-210-736-7229.

Plant Physiol. (1998) 116: 1505-1513
Copyright Clearance Center:   0032-0889/98/116/1505/09
© 1998 American Society of Plant Physiologists




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