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A Cyanobacterium Lacking Iron Superoxide Dismutase Is
Sensitized to Oxidative Stress Induced with Methyl Viologen but Is
Not Sensitized to Oxidative Stress Induced with
Norflurazon1
David J. Thomas,
Thomas J. Avenson,
Jannette B. Thomas, and
Stephen K. Herbert*
University of Idaho, Biological Sciences Department, Moscow, Idaho
83844-3051
A strain of
Synechococcus sp. strain PCC 7942 with no functional Fe
superoxide dismutase (SOD), designated
sodB , was characterized by its growth
rate, photosynthetic pigments, and cyclic photosynthetic electron
transport activity when treated with methyl viologen or norflurazon
(NF). In their unstressed conditions, both the
sodB and wild-type strains had similar
chlorophyll and carotenoid contents and catalase activity, but the wild
type had a faster growth rate and higher cyclic electron transport
activity. The sodB was very sensitive to
methyl viologen, indicating a specific role for the FeSOD in protection
against superoxide generated in the cytosol. In contrast, the
sodB mutant was less sensitive than the
wild type to oxidative stress imposed with NF. This suggests that the
FeSOD does not protect the cell from excited singlet-state oxygen
generated within the thylakoid membrane. Another up-regulated
antioxidant, possibly the MnSOD, may confer protection against NF in
the sodB strain. These results support the
hypothesis that different SODs have specific protective functions
within the cell.
1
This work was supported by the U.S. Department
of Agriculture Seed Grant (no. 93-37311-9445), the National Science
Foundation Experimental Program to Stimulate Competitive Research in
Idaho, and University of Idaho Seed Grants to S.K.H.
*
Corresponding author; e-mail skherbe{at}uidaho.edu; fax
1- 208-885-7905.
Plant Physiol. (1998) 116: 1593-1602
Copyright Clearance Center: 0032-0889/98/116/1593/10
© 1998 American Society of Plant Physiologists
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