Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (96)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Degenhardt, J.
Right arrow Articles by Kochian, L. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Degenhardt, J.
Right arrow Articles by Kochian, L. V.
Agricola
Right arrow Articles by Degenhardt, J.
Right arrow Articles by Kochian, L. V.

Aluminum Resistance in the Arabidopsis Mutant alr-104 Is Caused by an Aluminum-Induced Increase in Rhizosphere pH1

Jörg Degenhardt, Paul B. Larsen, Stephen H. Howell, and Leon V. Kochian*

United States Plant, Soil, and Nutrition Laboratory, United States Department of Agriculture-Agriculture Research Station, Tower Road, Cornell University, Ithaca, New York 14853 (J.D., P.B.L., L.V.K.); and Boyce Thompson Institute, Tower Road, Cornell University, Ithaca, New York 14853 (S.H.H.)

A mechanism that confers increased Al resistance in the Arabidopsis thaliana mutant alr-104 was investigated. A modified vibrating microelectrode system was used to measure H+ fluxes generated along the surface of small Arabidopsis roots. In the absence of Al, no differences in root H+ fluxes between wild type and alr-104 were detected. However, Al exposure induced a 2-fold increase in net H+ influx in alr-104 localized to the root tip. The increased flux raised the root surface pH of alr-104 by 0.15 unit. A root growth assay was used to assess the Al resistance of alr-104 and wild type in a strongly pH-buffered nutrient solution. Increasing the nutrient solution pH from 4.4 to 4.5 significantly increased Al resistance in wild type, which is consistent with the idea that the increased net H+ influx can account for greater Al resistance in alr-104. Differences in Al resistance between wild type and alr-104 disappeared when roots were grown in pH-buffered medium, suggesting that Al resistance in alr-104 is mediated only by pH changes in the rhizosphere. This mutant provides the first evidence, to our knowledge, for an Al-resistance mechanism based on an Al-induced increase in root surface pH.

1   This work was supported in part by the U.S. Environmental Protection Agency, Office of Research and Development (project no. R82-0001-010 to S.H.H. and L.V.K.).
*   Corresponding author; e-mail lvk1{at}cornell.edu; fax 1-607-255-2459.

Plant Physiol. (1998) 117: 19-27
Copyright Clearance Center:   0032-0889/98/117/0019/09
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. L. Yang, Y. Y. Li, Y. J. Zhang, S. S. Zhang, Y. R. Wu, P. Wu, and S. J. Zheng
Cell Wall Polysaccharides Are Specifically Involved in the Exclusion of Aluminum from the Rice Root Apex
Plant Physiology, February 1, 2008; 146(2): 602 - 611.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. M. Gabrielson, J. D. Cancel, L. F. Morua, and P. B. Larsen
Identification of dominant mutations that confer increased aluminium tolerance through mutagenesis of the Al-sensitive Arabidopsis mutant, als3-1
J. Exp. Bot., March 1, 2006; 57(4): 943 - 951.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. B. Kinraide, D. R. Parker, and R. W. Zobel
Organic acid secretion as a mechanism of aluminium resistance: a model incorporating the root cortex, epidermis, and the external unstirred layer
J. Exp. Bot., July 1, 2005; 56(417): 1853 - 1865.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. F. Ma, S. Nagao, C. F. Huang, and M. Nishimura
Isolation and Characterization of a Rice Mutant Hypersensitive to Al
Plant Cell Physiol., July 1, 2005; 46(7): 1054 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. J. Zheng, J. L. Yang, Y. F. He, X. H. Yu, L. Zhang, J. F. You, R. F. Shen, and H. Matsumoto
Immobilization of Aluminum with Phosphorus in Roots Is Associated with High Aluminum Resistance in Buckwheat
Plant Physiology, May 1, 2005; 138(1): 297 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Pineros, J. E. Shaff, H. S. Manslank, V. M. Carvalho Alves, and L. V. Kochian
Aluminum Resistance in Maize Cannot Be Solely Explained by Root Organic Acid Exudation. A Comparative Physiological Study
Plant Physiology, January 1, 2005; 137(1): 231 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J.-W. Pan, D. Ye, L.-L. Wang, J. Hua, G.-F. Zhao, W.-H. Pan, N. Han, and M.-Y. Zhu
Root Border Cell Development is a Temperature-Insensitive and Al-Sensitive Process in Barley
Plant Cell Physiol., June 15, 2004; 45(6): 751 - 760.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yamashita, T. Mimura, and K.-i. Shimazaki
Evidence for Nucleotide-Dependent Passive H+ Transport Protein in the Plasma Membrane of Barley Roots
Plant Cell Physiol., January 15, 2003; 44(1): 55 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Kobayashi and H. Koyama
QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana
Plant Cell Physiol., December 15, 2002; 43(12): 1526 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. J. Ahn, M. Sivaguru, G. C. Chung, Z. Rengel, and H. Matsumoto
Aluminium-induced growth inhibition is associated with impaired efflux and influx of H+ across the plasma membrane in root apices of squash (Cucurbita pepo)
J. Exp. Bot., September 1, 2002; 53(376): 1959 - 1966.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. F. Ma, R. Shen, Z. Zhao, M. Wissuwa, Y. Takeuchi, T. Ebitani, and M. Yano
Response of Rice to Al Stress and Identification of Quantitative Trait Loci for Al Tolerance
Plant Cell Physiol., June 15, 2002; 43(6): 652 - 659.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Tesfaye, S. J. Temple, D. L. Allan, C. P. Vance, and D. A. Samac
Overexpression of Malate Dehydrogenase in Transgenic Alfalfa Enhances Organic Acid Synthesis and Confers Tolerance to Aluminum
Plant Physiology, December 1, 2001; 127(4): 1836 - 1844.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Ezaki, M. Katsuhara, M. Kawamura, and H. Matsumoto
Different Mechanisms of Four Aluminum (Al)-Resistant Transgenes for Al Toxicity in Arabidopsis
Plant Physiology, November 1, 2001; 127(3): 918 - 927.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Wenzl, G. M. Patiño, A. L. Chaves, J. E. Mayer, and I. M. Rao
The High Level of Aluminum Resistance in Signalgrass Is Not Associated with Known Mechanisms of External Aluminum Detoxification in Root Apices
Plant Physiology, March 1, 2001; 125(3): 1473 - 1484.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
I. R. Silva, T. J. Smyth, D. F. Moxley, T. E. Carter, N. S. Allen, and T. W. Rufty
Aluminum Accumulation at Nuclei of Cells in the Root Tip. Fluorescence Detection Using Lumogallion and Confocal Laser Scanning Microscopy
Plant Physiology, June 1, 2000; 123(2): 543 - 552.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. Kollmeier, H. H. Felle, and W. J. Horst
Genotypical Differences in Aluminum Resistance of Maize Are Expressed in the Distal Part of the Transition Zone. Is Reduced Basipetal Auxin Flow Involved in Inhibition of Root Elongation by Aluminum?
Plant Physiology, March 1, 2000; 122(3): 945 - 956.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
V. Quesada, M. R. Ponce, and J. L. Micol
Genetic Analysis of Salt-Tolerant Mutants in Arabidopsis thaliana
Genetics, January 1, 2000; 154(1): 421 - 436.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
P. B. Larsen, J. Degenhardt, C.-Y. Tai, L. M. Stenzler, S. H. Howell, and L. V. Kochian
Aluminum-Resistant Arabidopsis Mutants That Exhibit Altered Patterns of Aluminum Accumulation and Organic Acid Release from Roots
Plant Physiology, May 1, 1998; 117(1): 9 - 17.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists