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Expression Pattern of the Carrot EP3
Endochitinase Genes in Suspension
Cultures and in Developing Seeds1
Arjon J. van Hengel2,
Flavia Guzzo3,
Ab van Kammen, and
Sacco C. de Vries*
Agricultural University Wageningen, Department of Molecular
Biology, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands
Carrot (Daucus carota)
extracellular protein 3 (EP3) class IV endochitinases were previously
identified based on their ability to rescue somatic embryos of the
temperature-sensitive cell line ts11. Whole-mount in situ hybridization
revealed that a subset of the morphologically distinguishable cell
types in embryogenic and nonembryogenic suspension cultures, including
ts11, express EP3 genes. No expression was found in
somatic embryos. In carrot plants EP3 genes are
expressed in the inner integumentary cells of young fruits and in a
specific subset of cells located in the middle of the endosperm of
mature seeds. No expression was found in zygotic embryos. These results
support the hypothesis that the EP3 endochitinase has a "nursing"
function during zygotic embryogenesis and that this function can be
mimicked by suspension cells during somatic embryogenesis.
1
This work was supported by The Netherlands
Organization for Scientific Research (A.J.v.H.) and by the European
Commission Biotechnology Program PTP-Biotech (F.G.).
2
Present address: Department of Cell Biology, John Innes
Centre, Colney Lane, Norwich NR4 7UH, UK.
3
Present address: Istituto Policattedra, Universita di
Verona, Verona 37134, Italy.
*
Corresponding author; e-mail sacco.devries{at}mac.mb.wau.nl; fax
31-317-483584.
Plant Physiol. (1998) 117: 43-53
Copyright Clearance Center: 0032-0889/98/117/0043/11
© 1998 American Society of Plant Physiologists
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