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Strontium-Induced Repetitive Calcium Spikes in a
Unicellular
Green Alga1
Claudia S. Bauer,
Christoph Plieth2,
Birgit Bethmann,
Ondina Popescu3,
Ulf-Peter Hansen,
Wilhelm Simonis, and
Gerald Schönknecht*
Botany I, University of Würzburg, Julius-von-Sachs Platz 2, D-97082 Würzburg, Germany (C.S.B., B.B., O.P., W.S., G.S.); and Institute for Applied Physics, University of Kiel, Olshausenstrasse
40, D-24098 Kiel, Germany (C.P., U.-P.H.)
The
divalent cation Sr2+ induced repetitive transient spikes of
the cytosolic Ca2+ activity
[Ca2+]cy and parallel repetitive transient
hyperpolarizations of the plasma membrane in the unicellular green alga
Eremosphaera viridis. [Ca2+]cy
measurements, membrane potential measurements, and cation analysis of
the cells were used to elucidate the mechanism of Sr2+-induced [Ca2+]cy
oscillations. Sr2+ was effectively and rapidly
compartmentalized within the cell, probably into the vacuole. The
[Ca2+]cy oscillations cause membrane
potential oscillations, and not the reverse. The endoplasmic reticulum
(ER) Ca2+-ATPase blockers 2,5-di-tert-butylhydroquinone and
cyclopiazonic acid inhibited Sr2+-induced repetitive
[Ca2+]cy spikes, whereas the
compartmentalization of Sr2+ was not influenced. A
repetitive Ca2+ release and Ca2+ re-uptake by
the ER probably generated repetitive [Ca2+]cy
spikes in E. viridis in the presence of
Sr2+. The inhibitory effect of ruthenium red and ryanodine
indicated that the Sr2+-induced Ca2+ release
from the ER was mediated by a ryanodine/cyclic ADP-ribose type of
Ca2+ channel. The blockage of Sr2+-induced
repetitive [Ca2+]cy spikes by
La3+ or Gd3+ indicated the necessity of a
certain influx of divalent cations for sustained
[Ca2+]cy oscillations. Based on these data we
present a mathematical model that describes the baseline spiking
[Ca2+]cy oscillations in E. viridis.
1
This work was financially supported by the
Deutsche Forschungsgemeinschaft within the SFB 176 (TP B11) and a
travel grant to O.P., and by a grant from the University of
Würzburg to C.S.B.
2
Present address: Institute of Cell and
Molecular Biology, The University of Edinburgh, Mayfield Road,
Edinburgh EH9 3JH, UK.
3
Present address: Anthropological Research Centre
of the Romanian Academy, Str. Eroilor no. 8, 762421 Bucharest 4, Romania.
*
Corresponding author; e-mail gerald{at}botanik.uni-wuerzburg.de;
fax 49-931-888-6158.
Plant Physiol. (1998) 117: 545-557
Copyright Clearance Center: 0032-0889/98/117/0545/13
© 1998 American Society of Plant Physiologists
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