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Overexpression of Iron Superoxide Dismutase in Transformed Poplar
Modifies the Regulation of Photosynthesis at Low CO2
Partial Pressures or Following Exposure to the Prooxidant Herbicide
Methyl Viologen1
Ana-Carolina M. Arisi,
Gabriel Cornic,
Lise Jouanin, and
Christine
H. Foyer*
Laboratoire de Biologie Cellulaire and Laboratoire du Metabolisme,
Institut National de la Recherche Agronomique, Route de Saint Cyr,
78026 Versailles cedex, France (A.-C.M.A., L.J.); Laboratoire
d'Ecologie Végétale, Groupe Photosynthèse et
Environnement, Unité de Recherche Associée, Centre National
de la Recherche Scientifique 2154, bât 362, Université de
Paris XI, F-91405 Orsay cedex, France (A.-C.M.A., G.C.); and Department
of Environmental Biology, Institute of Grassland and Environmental
Research, Plas Gogerddan, Ceredigion SY23 3EB, United Kingdom (C.H.F.)
Chloroplast-targeted overexpression
of an Fe superoxide dismutase (SOD) from Arabidopsis
thaliana resulted in substantially increased foliar SOD
activities. Ascorbate peroxidase, glutathione reductase, and
monodehydroascorbate reductase activities were similar in the leaves
from all of the lines, but dehydroascorbate reductase activity was
increased in the leaves of the FeSOD transformants relative to
untransformed controls. Foliar H2O2, ascorbate,
and glutathione contents were comparable in all lines of plants.
Irradiance-dependent changes in net CO2 assimilation and
chlorophyll a fluorescence quenching parameters were
similar in all lines both in air (21% O2) and at low (1%)
O2. CO2-response curves for photosynthesis showed similar net CO2-exchange characteristics in all
lines. In contrast, values of photochemical quenching declined in
leaves from untransformed controls at intercellular
CO2 (Ci) values below 200 µL L 1 but
remained constant with decreasing Ci in leaves of FeSOD transformants. When the O2 concentration was decreased from 21 to 1%, the
effect of FeSOD overexpression on photochemical quenching at limiting Ci was abolished. At high light (1000 µmol m2
s1) a progressive decrease in the ratio of variable
(Fv) to maximal (Fm) fluorescence was observed with
decreasing temperature. At 6oC the high-light-induced
decrease in the
Fv/Fm ratio was
partially prevented by low O2 but values were comparable in
all lines. Methyl viologen caused decreased
Fv/Fm ratios, but
this was less marked in the FeSOD transformants than in the
untransformed controls. These observations suggest that the rate of
superoxide dismutation limits flux through the Mehler-peroxidase cycle
in certain conditions.
1
A.-C.M.A. was supported by a doctoral fellowship
from Companha de Aperfeiçoamento de Pessoal de Nivel Superior,
Ministry of Education, Brazil.
*
Corresponding author; e-mail christine.foyer{at}bbsrc.ac.uk; fax
44-1970-828357.
Plant Physiol. (1998) 117: 565-574
Copyright Clearance Center: 0032-0889/98/117/0565/10
© 1998 American Society of Plant Physiologists
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