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The Role of Gibberellin, Abscisic Acid, and Sucrose in the
Regulation of Potato Tuber Formation in Vitro1
Xin Xu,
André A.M. van Lammeren,
Evert Vermeer, and
Dick Vreugdenhil*
Graduate School Experimental Plant Sciences, Department of Plant
Physiology (X.X., E.V., D.V.), and Department of Plant Cytology and
Morphology (X.X., A.A.M.v.L.), Wageningen Agricultural University,
Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
The effects of plant hormones and
sucrose (Suc) on potato (Solanum tuberosum L.)
tuberization were studied using in vitro cultured single-node cuttings.
Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus
gibberellin [GA]) media were tested. Tuberization frequencies, tuber
widths, and stolon lengths were measured during successive stages of
development. Endogenous GAs and abscisic acid (ABA) were identified and
quantified by high-performance liquid chromatography and gas
chromatography-mass spectrometry. Exogenous GA4/7 promoted
stolon elongation and inhibited tuber formation, whereas exogenous ABA
stimulated tuberization and reduced stolon length. Indoleacetic
acid-containing media severely inhibited elongation of stolons and
smaller sessile tubers were formed. Exogenous cytokinins did not affect
stolon elongation and tuber formation. Endogenous GA1 level
was high during stolon elongation and decreased when stolon tips
started to swell under inducing conditions, whereas it remained high
under noninducing conditions. GA1 levels were negatively
correlated with Suc concentration in the medium. We conclude that
GA1 is likely to be the active GA during tuber formation.
Endogenous ABA levels decreased during stolon and tuber development,
and ABA levels were similar under inducing and noninducing conditions.
Our results indicate that GA is a dominant regulator in tuber
formation: ABA stimulates tuberization by counteracting GA, and Suc
regulates tuber formation by influencing GA levels.
1
This work was supported by grants from the Royal
Dutch Academy of Sciences and from the Graduate School Experimental
Plant Sciences to X.X.
*
Corresponding author; e-mail dick.vreugdenhil{at}algem.pf.wau.nl;
fax 31-317-484740.
Plant Physiol. (1998) 117: 575-584
Copyright Clearance Center: 0032-0889/98/117/0575/10
© 1998 American Society of Plant Physiologists
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