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The Chloroplast atpA Gene Cluster in Chlamydomonas reinhardtii1
Functional Analysis of a Polycistronic Transcription Unit

Dominique Drapier, Hideki Suzuki2, Haim Levy3, Blandine Rimbault, Karen L. Kindle, David B. Stern*, and Francis-André Wollman

Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005, Paris, France (D.D., B.R., F.-A.W.); and Plant Science Center, Biotechnology Building (H.S., K.L.K.), and Boyce Thompson Institute for Plant Research (H.L., B.R., D.B.S.), Cornell University, Ithaca, New York 14853

Most chloroplast genes in vascular plants are organized into polycistronic transcription units, which generate a complex pattern of mono-, di-, and polycistronic transcripts. In contrast, most Chlamydomonas reinhardtii chloroplast transcripts characterized to date have been monocistronic. This paper describes the atpA gene cluster in the C. reinhardtii chloroplast genome, which includes the atpA, psbI, cemA, and atpH genes, encoding the alpha -subunit of the coupling-factor-1 (CF1) ATP synthase, a small photosystem II polypeptide, a chloroplast envelope membrane protein, and subunit III of the CF0 ATP synthase, respectively. We show that promoters precede the atpA, psbI, and atpH genes, but not the cemA gene, and that cemA mRNA is present only as part of di-, tri-, or tetracistronic transcripts. Deletions introduced into the gene cluster reveal, first, that CF1-alpha can be translated from di- or polycistronic transcripts, and, second, that substantial reductions in mRNA quantity have minimal effects on protein synthesis rates. We suggest that posttranscriptional mRNA processing is common in C. reinhardtii chloroplasts, permitting the expression of multiple genes from a single promoter.

1   This work was supported by the Centre National de la Recherche Scientifique (grant no. UPR9072 to F.-A.W.) and by the National Science Foundation (grant no. MCB 9406550 to K.L.K. and D.B.S.).
2   Present address: Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931.
3   Present address: Israeli Institute for Biological Research, P.O. Box 19, Nes-Ziona, Israel 70450.
*   Corresponding author; e-mail ds28{at}cornell.edu; fax 1-607-255-6695.

Plant Physiol. (1998) 117: 629-641
Copyright Clearance Center:   0032-0889/98/117/0629/13
© 1998 American Society of Plant Physiologists




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