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The Kinetics of Zeaxanthin Formation Is Retarded by Dicyclohexylcarbodiimide1

Sandra Heyde and Peter Jahns*

Heinrich-Heine-Universität Düsseldorf, Institut für Biochemie der Pflanzen, Universitätsstrasse 1, D-40225 Düsseldorf, Germany

The de-epoxidation of violaxanthin to antheraxanthin (Anth) and zeaxanthin (Zeax) in the xanthophyll cycle of higher plants and the generation of nonphotochemical fluorescence quenching in the antenna of photosystem II (PSII) are induced by acidification of the thylakoid lumen. Dicyclohexylcarbodiimide (DCCD) has been shown (a) to bind to lumen-exposed carboxy groups of antenna proteins and (b) to inhibit the pH-dependent fluorescence quenching. The possible influence of DCCD on the de-epoxidation reactions has been investigated in isolated pea (Pisum sativum L.) thylakoids. The Zeax formation was found to be slowed down in the presence of DCCD. The second step (Anth right-arrow Zeax) of the reaction sequence seemed to be more affected than the violaxanthin right-arrow Anth conversion. Comparative studies with antenna-depleted thylakoids from plants grown under intermittent light and with unstacked thylakoids were in agreement with the assumption that binding of DCCD to antenna proteins is probably responsible for the retarded kinetics. Analyses of the DCCD-induced alterations in different antenna subcomplexes showed that Zeax formation in the PSII antenna proteins was predominantly influenced by DCCD, whereas Zeax formation in photosystem I was nearly unaffected. Our data support the suggestion that DCCD binding to PSII antenna proteins is responsible for the observed alterations in xanthophyll conversion.

1   This work was supported by the Deutsche Forschungsgemeinschaft (grant no. SFB 189, TP B13).
*   Corresponding author; e-mail pjahns{at}uni-duesseldorf.de; fax 49-211-811-3706.

Plant Physiol. (1998) 117: 659-665
Copyright Clearance Center:   0032-0889/98/117/0659/07
© 1998 American Society of Plant Physiologists




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Greening under High Light or Cold Temperature Affects the Level of Xanthophyll-Cycle Pigments, Early Light-Inducible Proteins, and Light-Harvesting Polypeptides in Wild-Type Barley and the Chlorina f2 Mutant
Plant Physiology, May 1, 1999; 120(1): 193 - 204.
[Abstract] [Full Text]


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