Plant Physiol. Drug Metab Dispos
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Saline Stress Alters the Temporal Patterns of Xylem Differentiation and Alternative Oxidase Expression in Developing Soybean Roots1

Mirna Hilal, Ana M. Zenoff, Graciela Ponessa, Hortensia Moreno, and Eddy M. Massa*

Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Tecnológicas-Universidad Nacional de Tucumán), and Instituto de Química Biológica Dr. Bernabé Bloj, Chacabuco 461, San Miguel de Tucumán, 4000 Argentina (M.H., A.M.Z., H.M., E.M.M.); and Departamento de Morfología Vegetal, Fundación Miguel Lillo, M. Lillo 251, San Miguel de Tucumán, 4000 Argentina (G.P.)

We conducted a coordinated biochemical and morphometric analysis of the effect of saline conditions on the differentiation zone of developing soybean (Glycine max L.) roots. Between d 3 and d 14 for seedlings grown in control or NaCl-supplemented medium, we studied (a) the temporal evolution of the respiratory alternative oxidase (AOX) capacity in correlation with the expression and localization of AOX protein analyzed by tissue-print immunoblotting; (b) the temporal evolution and tissue localization of a peroxidase activity involved in lignification; and (c) the structural changes, visualized by light microscopy and quantified by image digitization. The results revealed that saline stress retards primary xylem differentiation. There is a corresponding delay in the temporal pattern of AOX expression, which is consistent with the xylem-specific localization of AOX protein and the idea that this enzyme is linked to xylem development. An NaCl-induced acceleration of the development of secondary xylem was also observed. However, the temporal pattern of a peroxidase activity localized in the primary and secondary xylem was unaltered by NaCl treatment. Thus, the NaCl-stressed root was specifically affected in the temporal patterns of AOX expression and xylem development.

1   This work was partially supported by the Consejo de Investigaciones de la Universidad Nacional de Tucumán and by the Consejo Nacional de Investigaciones Científicas y Tecnológicas of Argentina.
*   Corresponding author; e-mail massa{at}insibio.unt.edu.ar; fax 54-81-24-8025.

Plant Physiol. (1998) 117: 695-701
Copyright Clearance Center:   0032-0889/98/117/0695/07
© 1998 American Society of Plant Physiologists




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