|
Second Messengers Mediate Increases in Cytosolic Calcium in
Tobacco Protoplasts
Igor D. Volotovski,
Sergei G. Sokolovsky,
Olga V. Molchan, and
Marc
R. Knight1, *
Institute of Photobiology, Academy of Sciences of Belarus,
Academicheskaja Street 27, 220072 Minsk, Belarus (I.D.V., S.G.S.,
O.V.M.); and Department of Plant Sciences, University of Oxford, South
Parks Road, Oxford OX1 3RB, United Kingdom (M.R.K.)
Addition of membrane-permeable cyclic
GMP (cGMP) and cyclic AMP (cAMP) were shown to cause elevation of
cytosolic Ca2+ concentration
([Ca2+]cyt) in tobacco (Nicotiana
plumbaginofolia) protoplasts. Under the same conditions these
cyclic nucleotides were shown to provoke a physiological swelling
response in the protoplasts. Nonmembrane-permeable cAMP and cGMP were
unable to trigger a detectable [Ca2+]cyt
response. Cyclic-nucleotide-mediated elevations in
[Ca2+]cyt involved both internal and external
Ca2+ stores. Both cAMP- and cGMP-mediated
[Ca2+]cyt elevations could be inhibited by
the Ca2+-channel blocker verapamil. Addition of inhibitors
of phosphodiesterases (isobutylmethylxanthine and zaprinast) and the
adenylate cyclase agonist forskolin to the protoplasts (predicted to
elevate in vivo cyclic-nucleotide concentrations) caused elevations in
[Ca2+]cyt. Addition of the adenylate cyclase
inhibitor 2 ,5-dideoxyadenosine before forskolin significantly
inhibited the forskolin-induced [Ca2+]cyt
elevation. Taken together, these data suggest that a potential communication point for cross-talk between signal transduction pathways
using cyclic nucleotides in plants is at the level of Ca2+
signaling.
1
M.R.K. is a Royal Society University Research
Fellow.
*
Corresponding author; e-mail marc.knight{at}plants.ox.ac.uk; fax
44-1865-275023.
Plant Physiol. (1998) 117: 1023-1030
Copyright Clearance Center: 0032-0889/98/117/1023/08
© 1998 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
D. R Davies, L. V Bindschedler, T. S Strickland, and G P. Bolwell
Production of reactive oxygen species in Arabidopsis thaliana cell suspension cultures in response to an elicitor from Fusarium oxysporum: implications for basal resistance
J. Exp. Bot.,
May 1, 2006;
57(8):
1817 - 1827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Lemtiri-Chlieh and G. A. Berkowitz
Cyclic Adenosine Monophosphate Regulates Calcium Channels in the Plasma Membrane of Arabidopsis Leaf Guard and Mesophyll Cells
J. Biol. Chem.,
August 20, 2004;
279(34):
35306 - 35312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Cosio, E. Martinoia, and C. Keller
Hyperaccumulation of Cadmium and Zinc in Thlaspi caerulescens and Arabidopsis halleri at the Leaf Cellular Level
Plant Physiology,
February 1, 2004;
134(2):
716 - 725.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Becker, L. C. Boavida, J. Carneiro, M. Haury, and J. A. Feijo
Transcriptional Profiling of Arabidopsis Tissues Reveals the Unique Characteristics of the Pollen Transcriptome
Plant Physiology,
October 1, 2003;
133(2):
713 - 725.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ludidi and C. Gehring
Identification of a Novel Protein with Guanylyl Cyclase Activity in Arabidopsis thaliana
J. Biol. Chem.,
February 14, 2003;
278(8):
6490 - 6494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. P. Bolwell, L. V. Bindschedler, K. A. Blee, V. S. Butt, D. R. Davies, S. L. Gardner, C. Gerrish, and F. Minibayeva
The apoplastic oxidative burst in response to biotic stress in plants: a three-component system
J. Exp. Bot.,
May 15, 2002;
53(372):
1367 - 1376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sanders, J. Pelloux, C. Brownlee, and J. F. Harper
Calcium at the Crossroads of Signaling
PLANT CELL,
May 1, 2002;
14(90001):
S401 - 417.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Allen and J. I. Schroeder
Combining Genetics and Cell Biology to Crack the Code of Plant Cell Calcium Signaling
Sci. Signal.,
October 2, 2001;
2001(102):
re13 - re13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moutinho, P. J. Hussey, A. J. Trewavas, and R. Malho
cAMP acts as a second messenger in pollen tube growth and reorientation
PNAS,
August 17, 2001;
(2001)
171104598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Torrecilla, F. Leganés, I. Bonilla, and F. Fernández-Piñas
Use of Recombinant Aequorin to Study Calcium Homeostasis and Monitor Calcium Transients in Response to Heat and Cold Shock in Cyanobacteria
Plant Physiology,
May 1, 2000;
123(1):
161 - 176.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Q. Leng, R. W. Mercier, W. Yao, and G. A. Berkowitz
Cloning and First Functional Characterization of a Plant Cyclic Nucleotide-Gated Cation Channel
Plant Physiology,
November 1, 1999;
121(3):
753 - 761.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Moutinho, P. J. Hussey, A. J. Trewavas, and R. Malho
cAMP acts as a second messenger in pollen tube growth and reorientation
PNAS,
August 28, 2001;
98(18):
10481 - 10486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
