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The Biosynthesis of Salicylic Acid in Potato Plants1
Jean-Luc Coquoz,
Antony Buchala, and
Jean-Pierre Métraux*
Département de Biologie, Route Albert-Gockel 3, Université de Fribourg, CH-1700 Fribourg, Switzerland
Spraying potato (Solanum
tuberosum L.) leaves with arachidonic acid (AA) at 1500 µg
mL 1 led to a rapid local synthesis of salicylic acid (SA)
and accumulation of a SA conjugate, which was shown to be
2-O- -glucopyranosylsalicylic acid. Radiolabeling
studies with untreated leaves showed that SA was synthesized from
phenylalanine and that both cinnamic and benzoic acid were
intermediates in the biosynthesis pathway. Using radiolabeled
phenylalanine as a precursor, the specific activity of SA was found to
be lower when leaves were treated with AA than in control leaves.
Similar results were obtained when leaves were fed with the labeled
putative intermediates cinnamic acid and benzoic acid. Application of
2-aminoindan-2-phosphonic acid at 40 µM, an inhibitor of
phenylalanine ammonia-lyase, prior to treatment with AA inhibited the
local accumulation of SA. When the putative intermediates were applied
to leaves in the presence of 2-aminoindan-2-phosphonic acid, about 40%
of the expected accumulation of free SA was recovered, but the amount
of the conjugate remained constant.
1
This work was partially supported by the Swiss
National Science Foundation (grant no. 31-34098.92).
*
Corresponding author; e-mail jean-pierre.metraux{at}unifr.ch; fax
41-26-300-97-40.
Plant Physiol. (1998) 117: 1095-1101
Copyright Clearance Center: 0032-0889/98/117/1095/07
© 1998 American Society of Plant Physiologists
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