Plant Physiol.
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Dehydration-Stress-Regulated Transgene Expression in Stably Transformed Rice Plants1

Jin Su, Qingxi Shen, Tuan-Hua David Ho, and Ray Wu*

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853 (J.S., R.W.); and Plant Biology Program, Department of Biology, Washington University, St. Louis, Missouri 63130 (Q.S., T.-H.D.H.)

To confer abscisic acid (ABA) and/or stress-inducible gene expression, an ABA-response complex (ABRC1) from the barley (Hordeum vulgare L.) HVA22 gene was fused to four different lengths of the 5' region from the rice (Oryza sativa L.) Act1 gene. Transient assay of beta -glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1-100P) gives both the greatest induction and the highest level of absolute activity following ABA treatment. Two plasmids with one or four copies of ABRC1 combined with the same Act1-100P and HVA22(I) of barley HVA22 were constructed and used for stable expression of uidA in transgenic rice plants. Three Southern blot-positive lines with the correct hybridization pattern for each construct were obtained. Northern analysis indicated that uidA expression is induced by ABA, water-deficit, and NaCl treatments. GUS activity assays in the transgenic plants confirmed that the induction of GUS activity varies from 3- to 8-fold with different treatments or in different rice tissues, and that transgenic rice plants harboring four copies of ABRC1 show 50% to 200% higher absolute GUS activity both before and after treatments than those with one copy of ABRC1.

1   This work was supported by the Rockefeller Foundation (research grant no. RF93001, allocation no. 194) to R.W. J.S. was supported by a postdoctoral fellowship of the Rockefeller Foundation.
*   Corresponding author; e-mail ray.wu{at}cornell.edu; fax 1-607-255-2428.

Plant Physiol. (1998) 117: 913-922
Copyright Clearance Center:   0032-0889/98/117/0913/10
© 1998 American Society of Plant Physiologists




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