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A New Class of Arabidopsis Mutants with Reduced Hexadecatrienoic Acid Fatty Acid Levels1

Martine Miquel*, Claude Cassagne, and John Browse

Laboratoire Biogenèse Membranaire, Université Victor Segalen (Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5544), 146 rue Léo Saignat, Case 92, 33076 Bordeaux cedex, France (M.M., C.C.); and Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 (J.B.)

Chloroplast glycerolipids in a number of higher-plant species, including Arabidopsis thaliana, are synthesized by two distinct pathways termed the prokaryotic and eukaryotic pathways. The molecules of galactolipids produced by the prokaryotic pathway contain substantial amounts of hexadecatrienoic acid fatty acid. Here we describe a new class of mutants, designated gly1, with reduced levels of hexadecatrienoic acid. Lipid fatty acid profiles indicated that gly1 mutants exhibited a reduced carbon flux through the prokaryotic pathway that was compensated for by an increased carbon flux through the eukaryotic pathway. Genetic and biochemical approaches revealed that the gly1 phenotype could not be explained by a deficiency in the enzymes of the prokaryotic pathway. The flux of fatty acids into the prokaryotic pathway is sensitive to changes in glycerol-3-phosphate (G3P) availability, and the chloroplast G3P pool can be increased by exogenous application of glycerol to leaves. Exogenous glycerol treatment of gly1 plants allowed chemical complementation of the mutant phenotype. These results are consistent with a mutant lesion affecting the G3P supply within the chloroplast. The gly1 mutants may therefore help in determining the pathway for synthesis of chloroplast G3P.

1   This work was supported by the Centre National de la Recherche Scientifique and the Université Victor Segalen (Bordeaux, France), the National Science Foundation (grant no. IBN-9407902), and the Agricultural Research Center, Washington State University.
*   Corresponding author; e-mail miquel{at}biomemb.u-bordeaux2.fr; fax 33-5-5651-8361.

Plant Physiol. (1998) 117: 923-930
Copyright Clearance Center:   0032-0889/98/117/0923/08
© 1998 American Society of Plant Physiologists




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