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Transport of Sterols to the Plasma Membrane of Leek Seedlings1

Patrick Moreau*, Marie-Andrée Hartmann, Anne-Marie Perret, Bénédicte Sturbois-Balcerzak, and Claude Cassagne

Laboratoire de Biogenèse Membranaire, UMR 5544 Centre National de la Recherche Scientifique-Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France (P.M., A.-M.P., B.S.-B., C.C.); Institut de Biologie Moléculaire des Plantes (UPR Centre National de la Recherche Scientifique no. 406), Strasbourg, France (M.-A.H.); and Lipid and Lipoprotein Research Group, University of Alberta, Edmonton, Canada T6G252 (B.S.-B.)

To investigate the intracellular transport of sterols in etiolated leek (Allium porrum L.) seedlings, in vivo pulse-chase experiments with [1-14C]acetate were performed. Then, endoplasmic reticulum-, Golgi-, and plasma membrane (PM)-enriched fractions were prepared and analyzed for the radioactivity incorporated into free sterols. In leek seedlings sterols are present as a mixture in which (24R)-24-ethylcholest-5-en-3beta -ol is by far the major compound (around 60%). The other sterols are represented by cholest-5-en-3beta -ol, 24-methyl-cholest-5-en-3beta -ol, (24S)-24-ethylcholesta-5,22E-dien-3beta -ol, and stigmasta-5,24(241)Z-dien-3beta -ol. These compounds are shown to reside mainly in the PM. Our results clearly indicate that free sterols are actively transported from the endoplasmic reticulum to the PM during the first 60 min of chase, with kinetics very similar to that of phosphatidylserine. Such a transport was found to be decreased at low temperature (12°C) and following treatment with monensin and brefeldin A. These data are consistent with a membrane-mediated process for the intracellular transport of sterols to the PM, which likely involves the Golgi apparatus.

1   This work was supported by the Centre National de la Recherche Scientifique and the University Victor Segalen Bordeaux 2.
*   Corresponding author; e-mail pmoreau{at}biomemb.u-bordeaux2.fr; fax 33-05-56-51-83-61.

Plant Physiol. (1998) 117: 931-937
Copyright Clearance Center:   0032-0889/98/117/0931/07
© 1998 American Society of Plant Physiologists




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