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Chemotropic and Contact Responses of Phytophthora
sojae Hyphae to Soybean Isoflavonoids and Artificial
Substrates1
Paul F. Morris*,
Elizabeth Bone, and
Brett M. Tyler
Department of Biological Sciences, Bowling Green State University,
Bowling Green, Ohio 43403 (P.F.M., E.B.); and Department of Plant
Pathology, University of California, Davis, California 95616 (B.M.T.)
We have investigated the role of the
isoflavones daidzein and genistein on the chemotropic behavior of
germinating cysts of Phytophthora sojae. Hyphal
germlings were shown to respond chemotropically to daidzein and
genistein, suggesting that hyphal tips from zoospores that have
encysted adjacent to the root may use specific host isoflavones to
locate their host. Observations of the contact response of hyphal
germlings were made on several different substrates in the presence and
absence of isoflavones. Hyphal tips of germlings detected and
penetrated pores in membranes and produced multiple appressoria on
smooth, impenetrable surfaces. Hyphae that successfully penetrated the
synthetic membrane were observed to grow away from the membrane
surface. The presence of isoflavones in the medium surrounding the
hyphal germlings did not appear to alter any of those habits. Daidzein
and genistein did not inhibit germination or initial hyphal growth at
concentrations up to 20 µM.
1
This work was supported by U.S. Department of
Agriculture grant no. 94-37303-0700 to P.F.M. and B.M.T., and by
National Science Foundation equipment grants BIR-9009697 and
BIR-9249275.
*
Corresponding author; e-mail pmorris{at}bgnet.bgsu.edu; fax
1-419-372-2024.
Plant Physiol. (1998) 117: 1171-1178
Copyright Clearance Center: 0032-0889/98/117//08
© 1998 American Society of Plant Physiologists
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