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Decreased GA1 Content Caused by the Overexpression of
OSH1 Is Accompanied by Suppression of GA
20-Oxidase Gene Expression
Shinnosuke Kusaba*,
Masashi Fukumoto,
Chikako Honda,
Isomaro Yamaguchi,
Tomoaki Sakamoto, and
Yuriko Kano-Murakami
Division of Pomology, National Institute of Fruit Tree Science,
Tsukuba, Ibaraki 305-8605, Japan (S.K., M.F., C.H., Y.K.-M.); Faculty
of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032,
Japan (I.Y.); and Doctoral Program in Agricultural Science, University
of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan (T.S.)
We previously reported that
overexpression of the rice homeobox gene OSH1 led to
altered morphology and hormone levels in transgenic tobacco
(Nicotiana tabacum L.) plants. Among the hormones whose
levels were changed, GA1 was dramatically reduced. Here we
report the results of our analysis on the regulatory mechanism(s) of
OSH1 on GA metabolism. GA53 and
GA20, precursors of GA1, were applied
separately to transgenic tobacco plants exhibiting severely changed
morphology due to overexpression of OSH1. Only treatment with the end product of GA 20-oxidase, GA20, resulted in a
striking promotion of stem elongation in transgenic tobacco plants. The internal GA1 and GA20 contents in
OSH1-transformed tobacco were dramatically reduced
compared with those of wild-type plants, whereas the level of
GA19, a mid-product of GA 20-oxidase, was 25% of the
wild-type level. We have isolated a cDNA encoding a putative tobacco GA
20-oxidase, which is mainly expressed in vegetative stem tissue.
RNA-blot analysis revealed that GA 20-oxidase gene expression was
suppressed in stem tissue of OSH1-transformed tobacco plants. Based on these results, we conclude that overexpression of
OSH1 causes a reduction of the level of GA1
by suppressing GA 20-oxidase expression.
*
Corresponding author; e-mail kusa{at}fruit.affrc.go.jp; fax
81-298-38-6437.
Plant Physiol. (1998) 117: 1179-1184
Copyright Clearance Center: 0032-0889/98/117//06
© 1998 American Society of Plant Physiologists
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